Abstract： Objective Explore the clinical value of serum tumor markers,such as carbohydrate antigens(CA125), carcinoembryonic antigen(CEA), human epididymis protein 4 (HE4), malignant tumor specific growth factor (TSGF),combined with dynamic monitoring for the diagnosis of ovarian cancer. Methods From January 2011 to December 2013,100 cases of ovarian cancer patients (observation group) and 30 cases of patients with ovarian benign lesions (control group) were selectd as the research object, both of them by postoperative pathological diagnosis.Electrochemical luminescence method to detect patients with serum CA125, CEA, ELISA method for detecting HE4, chemical colorimetry to detect levels of TSGF, then analyse the correlation of ovarian benign and malignant tumors with serum levels of tumor molecular markers. Results CA125, CEA, HE4, TSGF level of ovarian cancer patients were obviously higher than that of ovarian benign tumor lesion group(P<0.01);Ovarian cancer is high ( Ⅲ , Ⅳ ) staging group was obviously higher than that of low stage group ( Ⅰ , Ⅱ ), lymph node metastasis were significantly higher than those without metastasis,recurrence were significantly higher than the relapse-free(P<0.01);One week after operation was significantly lower than that before operation(P<0.01);Four joint tumor molecular markers to detect the sensitivity and accuracy of the diagnosis of ovarian cancer, negative predictive value is significantly higher than individual test(P<0.01). Conclusion Serum CA125, CEA, HE4, TSGF levels associated with ovarian cancer clinical stage, recurrence and metastasis, the joint detection can improve the sensitivity and accuracy of the diagnosis of ovarian cancer, negative predictive value, at the same time,he joint dynamic monitoring to monitor treatment, recurrence and metastasis and follow-up have clinical significance.