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The mechanism of inhibitory effect of SJAMP on the proliferation in human pancreatic cancer SW1990's cells

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Author:
No author available
Journal Title:
Chinese Journal of Pancreatology
Issue:
1
DOI:
10.3760/cma.j.issn.1674-1935.2014.01.011
Key Word:
胰腺肿瘤;刺参属;黏多糖;Hippo信号通路;Pancreatic neoplasm;Stichopus;Mucopolysaccharides;Hippo pathway

Abstract´╝Ü Objective To investigate the possible mechanism of inhibitory effect of stichopus japonicus acidic mucopolysaccharide (SJAMP) on the proliferation in pancreatic carcinoma SW1990 cells.Methods SW1990 cells were incubated with different concentrations of SJAMP for 24,48,and 72 h.SW 1990 cells without SJAMP treatment were as control.RT-PCR was used to determine the expression of Hippo pathway members,including YAP,MST1 and related genes —— Survivin,TEAD1,Caspase-9,TEAD1 mRNA,and the protein expressions of YAP and phosphorylated YAP (p-YAP) in SW1990 cells were detected by Western blotting.Results The expression level in cells of control group was as 1,the YAP,TEAD1,Survivin,Caspase-9,MST1 mRNA expression after SJAMP treatment for 48 h were 0.27,0.31,0.26,4.06,7.06,and the expressions of YAP,TEAD1,Survivin mRNA were down-regulated,and the expressions of Caspase-9,MST1 mRNA were up-regulated in a time dependent manner,and the difference between the treatment group and control group was statistically significant (P <0.05 or P <0.01).The expressions of YAP,pYAD protein in control group were 0.705 ± 0.021,0.430 ± 0.043,and after SJAMP treatment for 48 h,the expressions were 0.545 ± 0.071,0.652 ± 0.025,and the expression of YAD was down-regulated,and the expression of pYAD was up-regulated in a time dependent manner,and the difference between the treatment group and control group was statistically significant (P <0.01).Conclutions SJAMP inhibits the proliferation of pancreatic carcinoma SW1990 cells possibly through Hippo pathway and relevant target genes.

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