Relationship between the expression of high mobility group protein B1 and blood-borne metastasis in pancreatic carcinoma
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PANCREATOLOGY
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.1674-1935.2008.05.008
- Key Word:
- 胰腺肿瘤;高迁移率族蛋白B1;血管;转移;Pancreatic neoplasm;High mobility group protein B1;Blood vessel;Metastasis
Abstract： Objective To investigate the correlation of the expression of high mobility group protein B1 (HMGB1) and blood-borne metastasis in patients with pancreatic carcinoma. Methods The serum HMGB1 levels were determined by Western blot analysis in 68 patients with pancreatic cancer, 18 patients with chronic pancreatitis and 21 healthy controls. Among the 68 patients with pancreatic cancer, the serum HMGB1 levels of 37 patients before and after operation were compared. The expression of HMGB1 and CD31 were detected by immunohistochemistry in 67 patients with pancreatic carcinoma. Results The serum HMGB1 levels in patients with pancreatic cancer was (119.7±54.5) ng/ml, which was significantly higher than (40.2±25.5) ng/ml in chronic pancreatitis (P<0.001) and (13.1±4.3) ng/ml in healthy control (P< 0.001). The serum HMGB1 levels in patients with pancreatic cancer before operation was (120.2±8.2) ng/ml, which was significantly higher than (69.3±5.1) ng/ml after operation (P <0.001). The expression rate of HMGB1 in pancreatic cancer tissues was 43.6%. The expression of HMGB1 were significantly related with tumor differentiation, TNM stage, blood-borne metastasis, and vascular density(P <0.01). The HMGB1 positive tumor cells were adjacent to the blood vessels with lumen, and the rate of HMGB1 expression in intravascular tumor cells was 71%. Conclusions The HMGB1 was highly expressed in pancreatic cancer tissues. HMGB1 expression positive pancreatic carcinoma cells were prone to invade blood vessels with lumen which may be related to blood-borne metastasis in pancreatic cancer.
- [1]王兴鹏,徐肇敏,许国铭.慢性胰腺炎诊治指南（2005年，南京）[J].2005,(5).
- [2]Riuzzi F,Donato R,Sorci G.The amphoterin (HMGB1)/receptor for advanced glycation end products (RAGE) pair modulates myoblast proliferation, apoptosis, adhesiveness, migration, and invasiveness. Functional inactivation of RAGE in L6 myoblasts results in tumor formation in vivo[J].2006,281(12).
- [3]Ochani M,Wang H,Sama AE,etc.IFN-gamma Induces High Mobility Group Box 1 Protein Release Partly Through a TNF-Dependent Mechanism.[J].2003,170(7).
- [4]Bonaldi T,Talamo F,Scaffidi P,etc.Monocytic cells hyperacetylate chromatin protein HMGB1 to redirect it towards secretion[J].2003,22(20).
- [5]H, Wang,O, Bloom,M, Zhang,etc.HMG-1 as a late mediator of endotoxin lethality in mice.[J].1999,285.
- [6]KVö,lp,M-LBrezniceanu,etc.Increased expression of high mobility group box 1 (HMGB1) is associated with an elevated level of the antiapoptotic c-IAP2 protein in human colon carcinomas[J].2006,55(2).
- [7]Palumbo R.,Bianchi ME.,Sampaolesi M.,etc.Extracellular HMGB1, a signal of tissue damage, induces mesoangioblast migration and proliferation[J].2004,164(3).
- [8]Ulloa L,Messmer D.High-mobility group box 1 (HMGB1) protein: friend and foe.[J].2006,17(3).
- [9]Wang H,Yang H,Tracey KJ.Extracellular role of HMGB1 in inflammation and sepsis.[J].2004,255(3).
- [10]Kuroda Y,Suzuki Y,Takada M,etc.Expression of receptor for advanced glycation end products (RAGE) and MMP-9 in human pancreatic cancer cells.[J].2004,51(58).
- [11]Yang L,Bruchfeld AN,Tracey KJ,etc.Elevated high-mobility group box 1 levels in patients with cerebral and myocardial ischemia.[J].2006,25(6).
- [12]Dumitriu IE,Manfredi AA,Baruah P,etc.HMGB1: guiding immunity from within.[J].2005,26(7).