The expression and effect of BMP-7 in rat with chronic pancreatitis
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PANCREATOLOGY
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.1674-1935.2007.06.012
- Key Word:
- 胰腺炎,慢性;骨形态发生蛋白质类;转化生长因子β
Abstract： 目的 研究骨形成蛋白-7(BMP-7)在大鼠CP组织中的表达,探讨其在胰腺纤维化形成中的意义.方法 每周2次腹腔内注射二乙基二硫代氨基甲酸盐(diethyldithiocarbamate, DDC)建立大鼠CP模型.HE染色观察正常组及造模后2、4、6、8周时大鼠胰腺病理学变化,VG染色观察胶原纤维含量,免疫组化检测BMP-7、TGF-β1、Smad2/3的表达.结果 (1)正常组BMP-7在腺泡细胞的胞质中表达.造模后4周腺泡细胞及部分炎性细胞BMP-7阳性面积比及阳性灰度值较正常组减少(P＜0.05).造模后8周腺体萎缩几乎无BMP-7表达,残留腺泡中表达量较正常胰腺显著减少[阳性面积比:(5.63±1.65)% vs (36.51±3.76)%, P＜0.01;灰度值:144.20±5.67 vs 174.54±1.22, P＜0.01].(2)TGF-β1和Smad2/3的表达在纤维化进展中变化一致.对照组小叶间结缔组织和部分腺泡间有少量表达.8周时小叶内纤维化区域及残留腺泡细胞间可见大量TGF-β1和Smad2/3表达,TGF-β1为(22.66±6.73)%;Smad2/3为(24.58±4.35)%,较正常组增加(P＜0.01).(3)TGF-β1与Smad2/3表达呈正相关(r = 0.61, P = 0.005), BMP-7与TGF-β1、Smad2/3表达均呈负相关(r值分别为-0.69和-0.68,P = 0.001).结论 胰腺纤维化过程中TGF-β1/Smad2/3信号通路活化,可促进细胞外基质沉积和腺泡细胞萎缩.BMP-7的表达量可反映残存腺泡细胞的数量,可能对CP的进展具有潜在的保护作用.
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