The effect of hypoxia on expression of integrinα5β1 in pancreatic carcinoma cell
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PANCREATOLOGY
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1674-1935.2007.03.014
- Key Word:
- 缺氧诱导因子1,α亚基;整合素α5β1;胰腺肿瘤;PANC-1
Abstract： 目的 探讨缺氧环境对人胰腺癌细胞株PANC1诱导因子-1α(hypoxia-inducible factor 1α,HIF-1α)、整合素α5(integrinα,INFα5)和整合素β1(integrinβ1,INTβ1)表达及侵袭力的影响.方法 以5%氧条件下培养PANC1,采用RT-PCR和Western blot 检测 HIF-1α、INTα5、的mRNA 和蛋白表达;采用Matrigel胶观测人胰腺癌细胞黏附侵袭能力的变化.并加入HIF-1α抑制剂YC-1缺氧培养PANC1,观察上述各指标的变化.结果 HIF-1α蛋白表达从常规培养时0.497±0.011逐渐上升到缺氧培养24h的1.455±0.133(P＜0.05),应用YC-1后蛋白表达下降到0.465±0.073(P＜0.05),而HIF-1α mRNA 表达无明显变化;INTα5蛋白从常规培养时0.964±0.032逐渐下降到缺氧培养24h的0.465±0.073(P＜0.05),应用YC-1后蛋白表达又上升到0.883±0.013(P＜0.05),INTα5mRNA的表达从0.212±0.023下降到0.018±0.002(P＜0.05),应用YC-1后再上升到0.069±0.011(P＜0.05);INTβ1的蛋白和mRNA 表达于缺氧培养及YC-1处理后均无明显变化.细胞侵袭力从常规培养时69.9±30.5逐渐上升到缺氧培养24h的105.0±14.9(P＜0.05),应用YC-1后又不降到79.7±20.9(P＜0.05).结论缺氧微环境下HIF-1α过表达可能通过下调INTα5来调控胰腺癌细胞间和细胞与基质间的黏附能力,有利于进一步侵袭转移.
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