You Position: Home > Paper
Experimental study on the effect of melatonin and Vitamin C on lipid peroxidization reaction in severe acute pancreatitis
( views:345, downloads:20 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PANCREATOLOGY
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1674-1935.2007.02.016
- Key Word:
- 胰腺炎,急性坏死性;褪黑激素;抗坏血酸;脂质过氧化作用
Abstract： 目的 观察褪黑素(MLT) 、维生素C(Vit C)在实验性急性坏死性胰腺炎(ANP)时对脂质过氧化反应的影响.方法 100只Wistar大鼠随机分为正常对照组(正常组)、ANP + 生理盐水组(ANP组)、ANP + MLT组(MLT组)、ANP + Vit C组(Vit C组)、ANP + MLT + Vit C组(联合组).采用胰胆管末端穿刺逆行注入3%牛磺胆酸钠(1 ml/kg体重)制备ANP模型.MLT组于制模后即刻皮下注射MLT 50 mg/kg体重,Vit C组肌内注射Vit C 1g/kg体重.联合组则合并应用.术后6 h和24 h处死大鼠,取血检测血清淀粉酶(AMY)、血清丙二醛(MDA)和超氧化物歧化酶(SOD)活性.胰腺病变程度按病理学评分,电镜观察超微结构.结果 MLT组、Vit C组和联合组在各时间点的AMY及MDA含量较ANP组显著降低(P ＜ 0.01),各时间点的SOD活性较ANP组显著升高(P ＜ 0.01).MLT组、Vit C组和联合组各时间点的胰腺炎症、腺泡坏死程度及出血均较ANP组明显减轻,病理分值明显低于ANP组(P ＜ 0.01或P ＜ 0.05).结论 自由基所引发的脂质过氧化反应损伤是SAP发病过程中的重要因素之一,MLT及Vit C可有效地减轻SAP时脂质过氧化反应损伤,明显减轻SAP时胰腺的病理改变,两者联合使用效果更佳.
- [1]杜卫东,沈达明,黄春锦,等.大剂量维生素C在急性胰腺炎患者脂质过氧化损伤中的保护作用[J].2002,(5).
- [2]Rojas C.,Mendez J.,Barja G.,etc.Endotoxin Depletes Ascorbate in the Guinea Pig Heart - Protective Effects of Vitamins C and E Against Oxidative Stress[J].1996,59(8).
- [3]F M, Abu-Zidan,M J, Bonham,J A, Windsor.Severity of acute pancreatitis: a multivariate analysis of oxidative stress markers and modified Glasgow criteria.[J].2000,87(8).
- [4]A U, Steinle,H, Weidenbach,M, Wagner,etc.NF-kappaB/Rel activation in cerulein pancreatitis.[J].1999,116.
- [5]陈敏.褪黑素与自由基[J].1999,(4).
- [6]J, Schmidt,D W, Rattner,K, Lewandrowski,etc.A better model of acute pancreatitis for evaluating therapy.[J].1992,215.
- [7]Reiter R.,Munozhoyos A.,Garcia JJ.,etc.PHARMACOLOGICAL ACTIONS OF MELATONIN IN OXYGEN RADICAL PATHOPHYSIOLOGY [Review][J].1997,60(25).
- [8]K Tsai,S-S Wang,T-S Chen,etc.Oxidative stress: an important phenomenon with pathogenetic significance in the progression of acute pancreatitis[J].1998,42(6).
- [9]Halangk W,Schulz HU,Klonowski Stumpe-H,etc.Oxidative stress in acute pancreatitis.[J].1999,46(29).