You Position: Home > Paper
Expressions of Caspase-1 activated cytokines in experimental severe acute pancreatitis
( views:212, downloads:9 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PANCREATOLOGY
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1674-1935.2003.04.005
- Key Word:
- 胰腺炎;Caspase-1;白细胞介素1;白细胞介素18;基因表达
Abstract： 目的研究实验性重症急性胰腺炎(SAP)Caspase-1激活的细胞因子的表达.方法SD大鼠32只,随机分4组:正常对照组(HC组)、SAP 6 h组、SAP 12 h组、SAP 18 h组.采用5%牛磺胆酸钠逆行注入胰胆管诱发大鼠SAP模型.通过HITACHI-7150型自动生化分析仪检测大鼠血清淀粉酶;采用ELISA法测定血清IL-1β水平;酶化学法测定胰腺MPO活性;半定量RT-PCR检测胰腺Caspase-1、IL-1β及IL-18 mRNA的表达;免疫组织化学方法检测胰腺IL-1β蛋白的表达.结果SAP各组血清淀粉酶和IL-1β水平显著升高,并伴有胰腺组织髓过氧化物酶(MPO)显著增加,与HC组相比,其差异均具有显著性意义(P＜0.01).HC组胰腺组织内可见Caspase-1 mRNA表达,但IL-1β及IL-18 mRNA的表达较弱SAP各组胰腺组织内Caspase-1、IL-1β及IL-18 mRNA的表达显著上调(P＜0.01).SAP各组IL-1β蛋白表达显著增强,与HC组比较差异具有显著意义(P＜0.01),而且与SAP严重程度相一致.结论Caspase-1激活的细胞因子IL-1β及IL-18的过度表达在SAP发病机制中发挥重要的作用.
- [1]Gregory W. Fink,James G. Norman.Intrapancreatic Interleukin-1β Gene Expression by Specific Leukocyte Populations during Acute Pancreatitis[J].1996,63(1).
- [2]Mayer J,Beger HG,Pralle U,etc.Natural course of acute pancreatitis.[J].1997,21(2).
- [3]Van der-Meer-JW,McIntyre RC Jr,Joosten LA,etc.Neutralization of IL-18 reduces neutrophil tissue accumulation and protects mice against lethal Escherichia coli and Salmonella typhimurium endotoxemia.[J].2000,164(5).
- [4]Fantuzzi G,Gu Y,Su MS,etc.Interleukin-18 (IFNgamma-inducing factor) induces IL-8 and IL-1beta via TNFalpha production from non-CD14+ human blood mononuclear cells.[J].1998,101(3).
- [5]W Denham,J Yang,G Fink,etc.Gene targeting demonstrates additive detrimental effects of interleukin 1 and tumor necrosis factor during pancreatitis[J].1997,113(5).
- [6]Dinarello CA.IL-18: A TH1-inducing, proinflammatory cytokine and new member of the IL-1 family.[J].1999,103(1 Pt.1).