You Position: Home > Paper
Tumor Suppressor Gene p16 Is Inactivated by Homozygous Deletion in Pancreatic Adenocarcinoma Cell Lines
( views:131, downloads:12 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PANCREATOLOGY
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.1674-1935.2001.01.012
- Key Word:
- 肿瘤抑制基因;p16;胰腺腺癌;纯合性缺失;甲基化
Abstract： 目的检测肿瘤抑制基因p16在胰腺腺癌细胞系中的基因改变和在mRNA以及蛋白质水平的表达.方法使用PCR方法检测基因的缺失情况;使用单链构象多态性(SSCP)方法检测基因有无突变;使用甲基化特异性PCR(MSP)方法检测基因启动子区域的甲基化状态;使用RT-PCR方法检测基因在mRNA水平的表达;使用免疫细胞化学染色和Western blot方法检测基因在蛋白质水平的表达.结果被检测的6个胰腺腺癌细胞系中,有3个细胞系存在p16基因的纯合性缺失,这3个细胞系在mRNA水平和蛋白质水平均无p16基因的表达.在全部6个细胞系中没有发现p16基因突变和启动子区域的过甲基化.结论在被检测的6个胰腺腺癌细胞系中,纯合性缺失是造成p16基因失表达的原因.
- [1]C, Caldas,S A, Hahn,L T, da Costa,etc.Frequent somatic mutations and homozygous deletions of the p16 (MTS1) gene in pancreatic adenocarcinoma.[J].1994,8(1).
- [2]M, Serrano,G J, Hannon,D, Beach.A new regulatory motif in cell-cycle control causing specific inhibition of cyclin D/CDK4.[J].1993,366(6456).
- [3]M Naumann,N Savitskaia,C Eilert,etc.Frequent codeletion of p16/MTS1 and p15/MTS2 and genetic alterations in p16/MTS1 in pancreatic tumors[J].1996,110(4).
- [4]T, Ueki,M, Toyota,T, Sohn,etc.Hypermethylation of multiple genes in pancreatic adenocarcinoma.[J].2000,60(7).
- [5]E, Rozenblum,M, Schutte,M, Goggins,etc.Tumor-suppressive pathways in pancreatic carcinoma.[J].1997,57(9).
- [6]崔全才,陈杰,顾长芳,等.一株人胰腺癌细胞系的建立及其特性[J].1992,(6).
- [7]Q, Liu,Y X, Yan,M, McClure,etc.MTS-1 (CDKN2) tumor suppressor gene deletions are a frequent event in esophagus squamous cancer and pancreatic adenocarcinoma cell lines.[J].1995,10(3).
- [8]J, Chen,T H, Liu,X Y, Guo,etc.Two new human exocrine pancreatic adenocarcinoma cell lines in vitro and in vivo.[J].1990,103.
- [9]L, Huang,T L, Goodrow,S Y, Zhang,etc.Deletion and mutation analyses of the P16/MTS-1 tumor suppressor gene in human ductal pancreatic cancer reveals a higher frequency of abnormalities in tumor-derived cell lines than in primary ductal adenocarcinomas.[J].1996,56(5).
- [10]A Kamb,NA Gruis,J Weaver-Feldhaus,etc.A cell cycle regulator potentially involved in genesis of many tumor types[J].1994,264 (5157).
- [11]Burger PC,Li Q,Ahuja N,etc.Methylation and silencing of the Thrombospondin-1 promoter in human cancer.[J].1999,18(21).
- [12]R E, Wilentz,J, Geradts,R, Maynard,etc.Inactivation of the p16 (INK4A) tumor-suppressor gene in pancreatic duct lesions: loss of intranuclear expression.[J].1998,58.
- [13]James G. Herman,Jeremy R. Graff,Sanna Myohanen.Methylation-specific PCR: A novel PCR assay for methylation status of CpG islands[J].1996,93(18).
- [14]M, Schutte,R H, Hruban,J, Geradts,etc.Abrogation of the Rb/p16 tumor-suppressive pathway in virtually all pancreatic carcinomas.[J].1997,57(15).