Abstract： Objective:To explore the efficacy and advantage of laparoscopic-assisted high ligation of varicocele in adolescents.Methods:From June 2019 to December 2020, 33 children(34 sides)of varicocele were recruited.The inclusion criteria were Dubin classification, symptoms and positive ultrasonic diagnosis; subclinical and secondary varicocele were the exclusion criteria.All of them underwent laparoscopic high ligation of spermatic vein assisted by methylene blue-assisted identification of spermatic lymphatic channel and papaverine-assisted identification of spermatic artery.Sex hormone, spermatic vein and testicular ultrasonography were examined before and 6 months after operation.The changes of testicular volume and sex hormone levels before and after operation were compared.SPSS 20.0 was utilized for processing the relevant data by t-test or rank sum test.Results:With rising varicocele grade, the level of follicle-stimulating hormone(FSH) spiked gradually while the levels of luteinizing hormone(LH) and testosterone(TT)declined gradually.The levels of FSH decreased significantly 6 months after operation while the levels of LH and TT rose in various degrees.No significant difference existed in sex hormone levels at 6 months after operation.There were differences in testicular volume before and after operation and among different grades.No recurrent varicocele or occurrence of hydrocele was detected by ultrasound.Conclusions:The change of postoperative sex hormone level and expanding testicular volume prove that highly selective laparoscopic high ligation of spermatic vein with methylene blue and papaverine for identifying and preserving spermatic lymphatic vessels and spermatic artery.It is an effective and safe treatment of varicocele in adolescents.And the technique of preserving spermatic lymphatic vessels and spermatic artery may avoid testicular atrophy and scrotal edema during other operations in adulthood.