Protective effect of microRNA-223 on neurological function in rats with acute cerebral infarction
- Author:
- No author available
- Journal Title:
- Prevention and Treatment of Cardio-Cerebral-Vascular Disease
- Issue:
- 1
- DOI:
- 10.3969/j.issn.1009-816x.2023.01.004
- Key Word:
- 急性脑梗死;微小RNA-223;神经功能;炎症因子;细胞
Abstract： 目的 探讨微小RNA(miR)-223对急性脑梗死大鼠神经保护作用.方法 清洁级雄性健康SD大鼠随机分为假手术组(S组)、模型组(M组)、对照序列组(NC组)和miR-223模拟物组(miR组),每组12只,构建缺血-再灌注大鼠模型,S组于缝皮后10 min时经侧脑室注入10μL的0.9％ 氯化钠注射液,M组、NC组和miR组于恢复血流灌注前15 min,以同样的方法分别将等量0.9％氯化钠注射液、含5 nmol对照序列或miR-223模拟物的0.9％氯化钠注射液注入.分别于建模后和建模7 d时,采用Zea-Longa评分标准对各组大鼠神经功能评分,酶联免疫吸附试验检测血清中炎症因子水平,TUNEL法检测脑组织中细胞凋亡,实时荧光定量PCR术检测脑组织中miR-223表达.结果 M组、NC组和miR组大鼠建模后和建模7 d时神经功能评分均高于S组(F=83.943、87.410,P<0.01),miR组大鼠建模7 d时神经功能评分低于M组和NC组(P<0.01);与S组相比,M组、NC组和miR组大鼠血清中白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)水平升高(F=16.085、52.672、27.393,P<0.01),与M组和NC组相比,miR组大鼠血清中IL-1β、IL-6和TNF-α水平降低(P<0.05);M组、NC组和miR组大鼠脑组织中细胞凋亡指数均高于S组(F=461.821,P<0.01),miR组大鼠脑组织中细胞凋亡指数低于M组和NC组(P<0.05);M组和NC组大鼠脑组织中miR-223表达量低于S组,而miR组高于S组,差异有统计学意义(F=328.334,P<0.01).结论 miR-223可能对脑缺血-再灌注损伤大鼠神经功能有保护作用,机制可能与其抑制炎症反应而减少细胞凋亡有关.
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