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A study on preparation of decellularized porcine liver extracellular matrix

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Journal Title:
Chinese Journal of Biomedical Engineering
Key Word:
脱细胞基质;聚乙二醇辛基苯基醚;十二烷基硫酸钠;Decellularized matrice;Polyethylene glycol octyl phenyl ether;Sodium dodecyl sulfate

Abstract´╝Ü Objective:To compare and analyze the protein retention in decellularized porcine liver extracellular matrice (ECMs) prepared via two methods, so as to further optimize the preparation process.Methods:Decellularized porcine liver ECM was prepared respectively by using either one of two surfactants, polyethylene glycol octyl phenyl ether (Triton X-100) and sodium dodecyl sulfate (SDS) , combined with enzymolysis by trypsin. Hematoxylin and eosin (HE) staining, immunohistochemistry, and biomass spectrometry were used to compare and analyze the components and their contents in the two ECMs. Thereby, we optimized the preparation process of ECM and discussed the underlying mechanism.Results:Either Triton X-100 or SDS was eligible for preparation of ECM that satisfies international standards. The ECM prepared using Triton X-100 showed better retention of collagens and growth factors, but more loss of glycosaminoglycan (GAG) compared with that prepared using SDS.Conclusion:Combination use of trypsin, aqueous ammonia and Triton X-100 for decellularization may lead to preparation of ECM that satisfies international standards, meanwhile with less loss of ECM proteins.

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