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Establishment, evaluation and short-term changes of organ functions in animal model of chylous ascites

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Journal Title:
Chinese Journal of Biomedical Engineering
Key Word:
乳糜腹;SD大鼠;动物模型;器官功能;精准外科;Chylous ascites;SD rats;Animal model;Organ function;Precision surgery

Abstract´╝Ü Objective:To explore a more effective localizing material for surgical use, establish an animal model of chylous ascites in rats based on our previous studies on this condition, and explore the microscopic changes in systems and organs that may possibly happen in the short term.Methods:Twenty male SD rats were randomized into the control group and experimental group ( n=10 each) . The rats were given a mixture of long- and medium-chain fat emulsion by gavage at 30 minutes before surgery to facilitate identification of lymphatic vessels during the operation. In the experimental group, the abdominal segment of thoracic duct was exposed near the diaphragmatic crus and ligated at the highest level; surfaces of the iliac vessels on both sides were rendered naked distal-near to the bifurcation and locally fenestrated to create a low pressure area. In the control group, ligation of lymphatic vessels at the highest level was performed only. The rats were given continuously gavage of fat emulsion to promote development of chylous ascites depending on the changes in abdominal contour. We observed the formation of ascites in animals, and examined the changes in blood biochemistry and pathophysiology of microstructure in multiple organs after formation of the chylous ascites. Results:In the experimental group, the abdomen of rats gradually enlarged starting from the first week after operation, and in the second week, formation of chylous ascites was confirmed by peritoneocentesis when the feeding was stopped. The success rate of modeling was 60% (6/10) . The rat models were in good performance as evaluated by nano carbon tracing. There were no significant differences in the percentage count of red blood cells and hemoglobin, white blood cells and lymphocytes, platelets and the levels of total bilirubin, direct bilirubin, indirect bilirubin, albumin, globulin between groups after modeling (all P>0.05) . Compared with the control group, the experimental group presented more significant vacuolization of hepatocytes in the short term accompanied by fatty degeneration and portal lymphocyte infiltration; lung interstitial thickening and perivascular protein exudation. There were no obvious changes in the microstructure of kidney, intestinal mucosa and spleen. Conclusion:The successful modeling of chylous ascites in animal may help further exploration on the use of novel materials in precise intraoperative localization of chylous ascites, and provides a platform for basic research of chylous ascites in future.

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