Abstract： Objective:To perform the proteomics profiles of fibroblasts in granulation tissue from patients with benign airway stenosis (BAS) and thereby investigate the pathogenesis of BAS.Methods:Granulation tissue from 5 patients with BAS (BAS group) and normal bronchial tissue from 3 patients with lung cancer (control group) were harvested for primary culture of fibroblasts. The profile of proteome expression was examined with isobaric tags for relative and absolute quantitation (iTRAQ) to screen for differentially expressed proteins (DEPs) . The Gene Ontology (GO) database, Kyoto Encyclopedia of Genes and Genomes (KEGG) database and Search Tool for Recurring Instances of Neighboring Genes (STRING) database were used for enrichment analysis of the functions, metabolic pathways and interactions of the differentially expressed proteins.Results:A total of 93 DEPs were screened, among them, 36 were significantly down-regulated, and 57 were significantly up-regulated, in fibroblasts of granulation tissue of BAS. The DEPs were mainly localized on cell membrane and in the extracellular domain, and were engaged in ion binding and metabolic activity. The major pathways involved were glycolysis-gluconeogenesis metabolism, extracellular matrix-receptor binding, phosphatidylinositol 3-kinase (PI3K) -AKT signaling, microbial metabolism and secondary metabolite synthesis in complex environments.Conclusion:Abnormalities in extracellular matrix-receptor binding, PI3K-AKT signaling pathway and metabolism in fibroblasts of granulation tissue in lesion of BAS may be related to occurrence and development of BAS.