Abstract： Objective:The purpose of this study was to investigate the feasibility of RhCE blood group genotyping by high-resolution melting curve (HRM) analysis.Methods:A total of 300 transfusion-na?ve patients were randomly included for serological RhCE phenotyping. From these, 30 samples comprising 10 each with phenotypes CCee (group 1) , CcEe (group 2) , and ccEE (group 3) were selected. The three groups were set as controls between one another. Genomic DNA was extracted, 3 primer pairs were designed, and then the RhCE blood group was further determined by HRM genotyping.Results:The HRM genotyping results of these 30 samples were in accordance with the phenotypes by serology. In HRM analysis, the primer pair 1 identified all samples with genotype CC primer pair 2 identified all samples with genotypes CC, Cc, and cc, and primer pair 3 identified all samples with genotypes Ee, in each group. After the PCR amplification products of group 1 was added to the experimental products of primer pair 3, a second HRM analysis identified all samples with genotypes EE, Ee, and ee in each group.Conclusion:Therefore, high-resolution melting curves can be used for genotyping of RhCE blood groups.