Abstract： Objective:To investigate the distribution of pathogenic bacteria causing nosocomial infection and drug-resistance in our intensive care unit (ICU) from 2018 to 2020.Methods:Included in this study were clinical data from 87 patients hospitalized in ICU of Meizhou People’s Hospital between January 2018 and June 2020, comprising 16 cases from surgical intensive care unit (SICU) , 4 from cardiac intensive care unit (CICU) , 4 from emergency intensive care unit (EICU) , 53 from general intensive care unit (GICU) , 9 from neurosurgery intensive care unit (NSICU) , and 1 from respiratory medical intensive care unit (RICU) . The rate of nosocomial infections, source of specimens, distribution of pathogenic bacteria, and detection yield of drug-resistant bacteria were analyzed.Results:From 2018 through 2020, the rates of nosocomial infections in ICUs of the hospital were 0.39%, 0.44%, and 0.30%, respectively, corresponding to an overall infection rate of 0.39%. The sources of specimen from 87 ICU patients with nosocomial infection mainly included sputum (45.98%) , urine (24.14%) , and blood (13.79%) . A total of 87 pathogen strains were identified, among which, Escherichia coli accounted for the highest proportion (43.68%, 38/87) , followed by Acinetobacter baumannii (24.14%, 21/87) . E. coli predominated in pathogens detected from SICU and GICU. All of 87 pathogen strains were drug-resistant, with ESBL-positive strains accounting for the highest detection yield, followed by extensively drug-resistant (XDR) strains. ESBL-positive strains contributed to the highest detection yield of drug-resistant bacteria from SICU, CICU, GICU, and NSICU. All isolated strains of E. coli and Klebsiella pneumoniae subsp. pneumoniae were ESBL-positive strains, and all of Staphylococcus aureus strains were MRSA-positive. In A. baumannii, XDR strains accounted for the highest detection yield of drug-resistance [71.43% (15/21) ], followed by multi-drug resistant (MDR) strains [19.05% (4/21) ]. Analysis of antibacterial use showed that piperacillin-tazobactam, cefuroxime, piperacillin-sulbactam were administered frequently in infections with A. baumannii, E. coli, and K. pneumoniae subsp. pneumoniae, whereas cefotaxime-sulbactam sodium and piperacillin-tazobactam were administered frequently in infections with Staph. aureus, piperacillin-tazobactam, imipenem-cilastatin, teicoplanin were administered frequently in infections with Pseudomonas aeruginosa. Conclusion:In ICUs of Meizhou People’s Hospital, nosocomial infection causing pathogens are dominated by Gram-negative bacteria, and ESBL-positive bacteria are the common drug-resistant strains.