Abstract： To investigate the epidemiological characteristics and drug resistance rates of clinically isolated Salmonella in Guangzhou Women and Children's Medical Center. A retrospective analysis was performed for 1022 cases of isolated clinically Salmonella in our hospital. All strains were subjected to serological typing and drug susceptibility tests. The findings showed that 1022 Salmonella cases were derived from feces and blood, identified predominantly in infants and young children under 3 years old, and frequently occurring in summer and autumn. A total of 45 serotypes were identified, and the highest detection rate was reported with Salmonella typhimurium (80.0%) . Drug susceptibility test showed that Salmonella had the most high-resistance to Ampicillin (85.9%) , and low-resistance to piperacillin/tazobactam, cefoperazone/sulbactam, and ciprofloxacin. The resistance rate of fecal isolates to the tested antibacterial drugs was higher than that of blood isolates. The five-year mean detection rate of multi-drug resistant Salmonella (MDR) was 8.9%, among which Salmonella typhimurium had the lowest multi-drug resistance rate (6.8%) . In conclusion, antibacterials in children should be carefully selected according to the serotype and drug sensitivity results.