You Position: Home > Paper

Enteral immune-microecological nutrition support suppresses inflammatory response in rats with acute pancreatitis

( views:0, downloads:0 )
Author:
No author available
Journal Title:
Chinese Journal of Biomedical Engineering
Issue:
5
DOI:
10.3760/cma.j.cn115668-20210207-00022
Key Word:
肠内免疫微生态营养;Toll样受体4;髓样分化因子88;急性胰腺炎;炎症反应;Enteral immune microecological nutrition;Toll-like receptor 4;Myeloid differentiation factor 88;Acute pancreatitis;Inflammatory response

Abstract´╝Ü Objective:To investigate the effect of enteral immune-microecological nutrition (EIN) nutritional support on inflammatory response in rats with acute pancreatitis and its mechanism.Methods:Thirty-two male Sprague Dawley rats were randomly divided into the control group, model group, enteral nutrition (EN) group and EIN group. The model of acute pancreatitis was established in the model group, EN group and EIN group. The control group and model group were not intervened, while the EN group and EIN group were intervened with EN and EIN protocols, respectively. After the intervention, the rats were harvested for pancreatic glands which were then analyzed for pathological characteristics by HE staining. The blood levels of amylase (AMY) , pancrelipase (LPS) , calcium (Ca 2+) , tumor necrosis factor (TNF-α) and Interleukin 1β (IL-1 β) were measured. The mRNA and protein levels of Toll-like receptor 4 (TLR4) , myeloid differentiation factor 88 (MyD88) and β - actin were examined by PCR and Western blotting. Results:In the EIN group, the pancreatic lobule showed destructed structure uder microscopy, with purplish red necrotic foci, interstitial hyperemia and edema, and a small amount of inflammatory cells infiltration in the necrotic area. After intervention, there were significant differences in AMY, LPS, Ca 2+, TNF-α, and IL-1β across the control group, model group, EN group and EIN group. Among them, the Ca 2+ level was lowest in the model group, and was significantly higher in the EIN group compared with the model group and the EN group ( P<0.05) . There were also significant differences in TLR4 and MyD88 mRNA and protein expression across the control group, model group, EN group and EIN group. These expression levels were highest in the model group, and was significantly lower in the EIN group compared with the model group and EN group ( P<0.05) . Conclusion:EIN nutritional support can effectively inhibit the inflammatory response in rats with acute pancreatitis via the TLR4/MyD88 pathway and thereby reduce the expression of inflammatory factors.

WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn