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Effect of miR-92a on podocyte injury induced by high glucose and its mechanism
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- Author:
- No author available
- Journal Title:
- Chinese Journal of Biomedical Engineering
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.1674-1927.2019.06.009
- Key Word:
- 足细胞;高糖;微RNA-92a;细胞凋亡;上皮间质转化;Smad7
Abstract： 目的 探讨微RNA(miR)-92a对高糖诱导的足细胞损伤的影响及其机制.方法 将体外培养的足细胞分为对照组(未处理)、高糖组(高糖处理)、高糖+miR-NC组(高糖环境下转染miR-92a阴性对照)和高糖+miR-92a组(高糖环境下转染miR-92a模拟物),采用RT-PCR检测各组细胞中miR-92a的表达,流式细胞仪检测细胞凋亡,Caspase-3活性检测试剂盒检测Caspase-3活性,免疫印迹检测足细胞损伤标志蛋白Podocin、Nephrin、Desmin和上皮间质转化相关蛋白E-cadherin、α-SMA、Smad7的表达情况.双荧光素酶报告基因实验检测miR-92a与Smad7的靶向关系.结果 与对照组相比,高糖组、高糖+miR-NC组和高糖+miR-92a组细胞中miR-92a和Desmin、α-SMA蛋白的表达水平以及细胞凋亡率、Caspase-3活性均明显升高,而Podocin、Nephrin、E-cadherin和Smad7蛋白的的表达水平明显降低(P<0.05),且高糖+miR-92a组变化幅度明显大于高糖组(P<0.05),而高糖+miR-NC组和高糖组之间差异无统计学意义(P>0.05).双荧光素酶报告基因实验证实Smad7是miR-92a的靶基因.结论 miR-92a可通过促进足细胞凋亡和上皮间质转化加重高糖诱导的足细胞损伤,其作用机制可能与靶向调控Smad7表达有关.
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