Abstract： Objective To investigate the anti-inflammatory effect of choline supplementation in rats with pre-eclampsia (PE) during pregnancy. Methods PE model was established in rats at 14d of pregnancyby slowly pumping extremely low dose of endotoxin(LPS,1.0 ug/kg)via the caudal vein. Thirty rats were randomly divided into 5 groups,including the normal non-pregnant rats group(NP),normal pregnant rats group(P),normal pregnant rats supplemented with choline diet group(P+C),PE group (LPS),and PE rats supplemented with choline diet group(LPS+C),with 6 rats in each group. The systolic blood pressure(SBP),24 h urinary albumin,and liver and kidney function were determined in each group. Multi-functional liquid phase chip analysis was used to determine the levels of inflammatory cytokines in serum and placenta at 20 d of pregnancy in the pregnant rats. Results After the injection with endotoxin, the levels of SBP and urinary albumin in the LPS group significantly increased,whereas the levels of SBP and urinary albumin in the LPS+C group decreased compared with the LPS group. The serum levels of proinflammatory cytokines TNF-α,IL-1β,IL-6,IFN-γ,GM-CSF and IL-17A in endotoxin-induced PE rat models significantly increased. The placenta levels of proinflammatory cytokines TNF-α,IL-1β,IL-2,IL-6, GM-CSF and IL-17A significantly increased,whereas the level of anti-inflammatory cytokine IL-4 in the serum and placenta decreased. After supplemented with choline,the levels of proinflammatory cytokines TNF-α,IFN-γ,IL-1β,IL-6 and IL-17A in the serum decreased. The levels of proinflammatory cytokines TNF-α,IL-1β,IL-6 and IL-17A in the placenta significantly increased,whereas the level of anti-inflammatory cytokine IL-4 in the serum and placenta significantly increased. Conclusion Choline supplementation during pregnancy may effectively improve symptoms and inhibit the systemic and placental inflammatory response in PE rats.