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Expression of mammalian target of rapamycin in hippocampal area of Fmr1 knockout mice
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- Author:
- No author available
- Journal Title:
- Chinese Journal of Biomedical Engineering
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1674-1927.2018.02.005
- Key Word:
- 脆性X综合征;基因敲除;哺乳动物雷帕霉素靶蛋白;Fmr1
Abstract： 目的 检测4周龄Fmr1基因敲除(KO)小鼠海马组织中哺乳动物雷帕霉素靶蛋白(mTOR)的表达.方法 使用PCR技术选取4周龄Fmr1 KO小鼠和野生型(WT)小鼠各20只,免疫印迹法检测小鼠海马组织中mTOR、磷酸化mTOR(p-mTOR)的表达.结果 免疫印迹显示,Fmr1 KO小鼠海马组织mTOR、p-mTOR相对表达量较WT小鼠均显著增加,差异具有统计学意义(均P<0.05).结论 海马组织mTOR表达增加可能与Fmr1 KO小鼠的脆性X综合征发病有关.
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