Effects of low expression of CD74 on proliferation,migration and invasion of human pancreatic cancer cells
- Author:
- No author available
- Journal Title:
- Chinese Journal of Biomedical Engineering
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.1674-1927.2017.05.002
- Key Word:
- 胰腺癌;抗原;CD74;细胞增殖;细胞迁移;侵袭;Capan-2细胞系;Pancreatic cancer;Antigens,CD74;Cell proliferation;Cell migration;Invasiveness;Capan-2 cell lines
Abstract： Objective To investigate the effect of interfering CD74 expression on the biological behavior of human pancreatic cancer cell line Capan-2. Methods The CD74-short hairpin RNA(CD74-shRNA)interference sequence was designed to construct CD74 interference plasmid transfected Capan-2 cells(group CD74-shRNA). The transfected pSUPER empty vectors were included as the controls. MTT assay was used to determine the cell proliferation activity.The scratch assay and Transwell assay were used to determine the cell migration and invasion.The real-time quantitative PCR and Western blotting were used to examine CD74 mRNA and protein expression. The real-time quantitative PCR was used to examine the mRNA expression of matrix metalloproteinase 2(MMP-2)and MMP-9. Results The cell proliferation activity test and scratch test showed that interfering CD74 expression could decrease Capan- 2 cell proliferation and inhibit cell migration(all P<0.05). The Transwell assay showed that the number of transmembrane cells in the CD74-shRNA group and the control group after culturing for 48 h were(102.5± 9.03)/well and(139.1 ± 9.89)/well,respectively,and the number in the CD74 - shRNA group was significantly lower than that in the control group(P<0.05).Compared with the control group,the inhibition rates of CD74 mRNA and protein expression in the CD74-shRNA group were 61% and 90%,respectively, and the inhibition rates of MMP-2 and MMP-9 mRNA expression in the CD74-shRNA group were 84% and 85%,respectively. Conclusion The low expression of CD74 can inhibit the proliferation,migration and invasion of Capan-2 cells.CD74 may serve as a new target for the treatment of pancreatic cancer.
- [1]黄耀星,贾林,王康玮,等.CD74在胰腺癌中的表达及与神经浸润的关系[J].2013,(24).
- [2]Ying-Hui Zhi,Mao-Min Song,Pi-Lin Wang,etc.Suppression of matrix metalloproteinase-2 via RNA interference inhibits pancreatic carcinoma cell invasiveness and adhesion[J].2009,(9).
- [3].Effect of CD74 on the prognosis of patients with resectable pancreatic cancer[J].2014,(1).
- [4]黄耀星,贾林,周荣佳,等.靶向CD74基因RNA干扰慢病毒稳定感染人胰腺癌细胞株的建立与鉴定[J].2016,(13).
- [5]Borghese F,Clanchy FI.CD74: an emerging opportunity as a therapeutic target in cancer and autoimmune disease.[J].2011,15(3).
- [6]David V, Gold,Rhona, Stein,Jack, Burton,etc.Enhanced expression of CD74 in gastrointestinal cancers and benign tissues.[J].2010,4(1).
- [7]Schroeder, Bernd.The multifaceted roles of the invariant chain CD74-More than just a chaperone[J].2016,1863(6 Pt.A).
- [8]Zervos EE,Rosemurgy AS 2nd,Bloomston M.Matrix metalloproteinases and their role in pancreatic cancer: a review of preclinical studies and clinical trials.[J].2002,9(7).
- [9]Delohery T,Fang Y,Xu J,etc.MIF Signal Transduction Initiated by Binding to CD74.[J].2003,197(11).
- [10]Pryczynicz A,Czyzewska J,Guzinska-Ustymowicz K,etc.Expression of matrix metalloproteinase 9 in pancreatic ductal carcinoma is associated with tumor metastasis formation.[J].2007,45(1).
- [11]Meyer-Siegler KL,Leng L,Iczkowski KA,etc.Inhibition of macrophage migration inhibitory factor or its receptor (CD74) attenuates growth and invasion of DU-145 prostate cancer cells.[J].2006,177(12).
- [12]Yadav,D.,Lowenfels,A.B..The epidemiology of pancreatitis and pancreatic cancer[J].2013,144(6).
- [13]Cao D,Yeo CJ,Hruban RH,etc.Differentially expressed genes in pancreatic ductal adenocarcinomas identified through serial analysis of gene expression.[J].2004,3(12).
- [14]Nagata S,Jin YF,Yoshizato K.CD74 is a novel prognostic factor for patients with pancreatic cancer receiving multimodal therapy.[J].2009,16(9).
- [15]Naama Gil-Yarom,Lihi Radomir,Lital Sever,etc.CD74 is a novel transcription regulator[J].2017,114(3).
- [16]Su, Huiting,Na, Ning,Zhang, Xiaodong,etc.The biological function and significance of CD74 in immune diseases[J].2017,66(3).
- [17]Gholaman H,Bruderek K,Whiteside TL,etc.Tumor-derived macrophage migration inhibitory factor modulates the biology of head and neck cancer cells via neutrophil activation.[J].2011,129(4).
- [18]Bacher M,Lan HY,Huang XR,etc.Macrophage migration inhibitory factor induces MMP-9 expression in macrophages via the MEK-ERK MAP kinase pathway.[J].2007,27(2).
- [19]Yasuhiro, Tamori,Xiaodong, Tan,Kazuhiro, Nakagawa,etc.Clinical significance of MHC class II-associated invariant chain expression in human gastric carcinoma.[J].2005,14(4).