Abstract： Objective To investigate the distribution and drug resistance of bacteria causing urinary tract infection in our hospital,and to provide evidence for rational clinical use of antibiotics. Methods The distribution and drug resistance of the positive bacteria isolates from mid?stream urine specimens of patients with urinary tract infection,who were hospitalized in our hospital between June 2012 and December 2014,were analyzed retrospectively. Susceptibility testing of common bacteria were compared and statistically analyzed. Results A total of 2 986 strains were isolated and cultured,including Gram?negative bacilli(GNB)(1 885,63.13%),Gram?positive cocci(GPC)(624,20.90%)and fungi(454, 15.20%). The GNB strains were mainly consisted of E. coli(1 199,40.15%)and Klebsiella pneumoniae (188,6.30%),producing extended spectrum beta?lactamases (ESBLs)[(623,51.96%) and (95, 50.53%),respectively. The GPC strains were consisted of enterococcus faecalis(202,6.76%),enterococcus faecium (190,6.36%) and staphylococcus (188,6.30%). The fungi strains were mainly consisted of candida(449,15.04%)and saccharomycetes(5,0.17%). The E. coli and Klebsiella pneumoniae strains producing ESBLs in urinary tract infection were sensitive to Imipenem, Meropenem, Amikacin and Cefoxitin,but were highly resistant to Quinolones. Most GPC strains were sensitive to Vancomycin, Teicoplanin and Linezolid,while fungi strains were sensitive to 5?Fluorocytosine,Voriconazole,Fluconazole and Amphotericin B. Conclusion GNB,with a variety of strains and a high antibiotic resistance rate,were the most common pathogens in urinary tract infection. Rational use of antibiotics should be paid high attention,in order to prevent abuse of antibiotics and production of new drug?resistant strains.