Gamma-H2AX:a novel quantitative biomarker of DNA damage after CT radiation exposure
- Author:
- No author available
- Journal Title:
- 中华生物医学工程杂志
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.1674-1927.2016.01.003
- Key Word:
- 体层摄影术,X线计算机;γ-H2AX;DNA损伤;Tomography,X-ray computed;γ-H2AX;DNA damage
Abstract： Objective To explore correlation between CT radiation dose and the number of gamma?H2AX (phosphorylated form of H2AX histone variant) foci in human peripheral blood mononuclear cells (PBMC)by usingγ?H2AX?based immunofluorescent assay. Methods 64 volunteers received phlebotomy for peripheral blood samples from cubital vein. Based on CT dose,a total of 16 groups of blood samples were used for in?vitro CT radiation (n=4 samples each). We measured the expression of PBMC γ?H2AX. Fluorescence microscopy was used for countingγ?H2AX foci. Results After in?vitro CT radiation,there was a significant increase in human PBMCγ?H2AX foci which was in positive linear correlation with the CT dose?length product(DLP)(R2=0.9357,P<0.05). Conclusion γ?H2AX quantitatively reflects CT radiation?induced peripheral blood DNA damage,which is justified for widespread use as a biomarker for low?dose ionizing radiation.
- [1]McNitt Gray MF.AAPM/RSNA Physics Tutorial for Residents: Topics in CT: Radiation Dose in CT.[J].2002,22(6).
- [2]Laurence Beels,Klaus Bacher,Peter Smeets,etc.Dose-length product of scanners correlates with DNA damage in patients undergoing contrast CT[J].2012,81(7).
- [3]Rogakou EP,Pilch DR,Orr AH,etc.DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139.[J].1998,273(10).
- [4]Balroop S,Shekhdar J,Goh V,etc.Leukocyte DNA damage after multi-detector row CT: a quantitative biomarker of low-level radiation exposure.[J].2007,242(1).
- [5]Kuo LJ,Yang LX.Gamma-H2AX - a novel biomarker for DNA double-strand breaks.[J].2008,22(3).
- [6]Lobrich M,Shibata A,Beucher A,etc.gammaH2AX foci analysis for monitoring DNA double-strand break repair: strengths, limitations and optimization.[J].2010,9(4).
- [7]Asako, Nakamura,Olga A, Sedelnikova,Christophe, Redon,etc.Techniques for gamma-H2AX detection.[J].2006,409.
- [8]Helen C. Turner,P. Sharma,J. R. Perrier.The RABiT: high-throughput technology for assessing global DSB repair[J].2014,53(2).
- [9]Philippe Voisin,Laurence Roy,Marc Benderitter.Why Can't We Find a Better Biological Indicator of Dose?[J].2004,112(4).
- [10]Nickoloff EL,Boone JM,Huda W.Overview of patient dosimetry in diagnostic radiology in the USA for the past 50 years.[J].2008,35(12).
- [11]Heckmann M,Uder M,Rief N,etc.In vivo formation and repair of DNA double-strand breaks after computed tomography examinations[J].2005,102(25).
- [12]Rothkamm K,Lobrich M.Evidence for a lack of DNA double-strand break repair in human cells exposed to very low x-ray doses.[J].2003,100(9).
- [13]Achenbach S,Lell M,Grudzenski S,etc.Effect of CT scan protocols on x-ray-induced DNA double-strand breaks in blood lymphocytes of patients undergoing coronary CT angiography.[J].2010,20(12).
- [14]Eileen Pernot,Janet Hall,Sarah Baatout,etc.Ionizing radiation biomarkers for potential use in epidemiological studies.[J].2012,751(2).
- [15]Juliane, Heydenreich,Christoph, Otto,Frank, Mayer,etc.Reliability of a Fully Automated Interpretation of γ -H2AX Foci in Lymphocytes of Moderately Trained Subjects under Resting Conditions.[J].2014,2014.