Expression of heat shock protein 60 and its correlation with germ cells apoptosis in testis of rats with varicocele
- Author:
- No author available
- Journal Title:
- Chinese Journal of Biomedical Engineering
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.1674-1927.2015.06.005
- Key Word:
- 精索静脉曲张;热休克蛋白质60;睾丸;细胞凋亡;Varicocele;Heat shock protein 60;Testis;Apoptosis
Abstract： Objective To investigate the expression of heat shock protein 60 (HSP60) and apoptosis related protein caspase 3 in testis of rat varicocele (VC),and the role of HSP60 in apoptosis of testicular germ cells in rats.Methods Thirty-two SD rats were randomly divided into the control group (n=8),sham operation group (n=8) and VC model group (n=16).VC rat models were prepared by ligation of the left renal vein.Real-time quantitative PCR and Western blot assay were used to determine the mRNA and protein expression levels of HSP60 in testicular tissues of VC rats;immunoblot assay was used to determine the expression of caspase 3 (activated type) in VC rat testis.We analyzed the relationship between HSP60 and caspase 3 expressions in VC rat testis.Results Compared with the control group and the sham operation group,VC rats had increased testis mRNA and protein levels of HSP60 and caspase 3 (HSP60 mRNA:2.67± 0.42 vs 1.18±0.30,0.98±0.16,HSP60 protein:12.02±1.78 vs 2.31±0.34,3.15±0.60;caspase 3 mRNA:4.28±0.47 vs 1.45±0.17,1.38±0.24,caspase 3 protein:5.44±2.06 vs 1.32±0.84,1.16±0.76,all P＜0.05).The difference was not statistically significant between sham operation group and the control group (all P＞ 0.05).In the VC model group,HSP60 and cleaved caspase 3 expression in rat testis tissue were positively correlated (R=0.95,P＜0.05).Conclusion HSP60 expression is increased in testis of VC rats,and may be involved in testicular germ cell apoptosis,which in turn essentially contributes to male infertility in VC.
- [1]Michael R. Elliott,Kodi S. Ravichandran.ELM01 signaling in apoptotic germ cell clearance and spermatogenesis[J].2010,1209.
- [2]Ricardo, Miyaoka,Sandro C, Esteves.A critical appraisal on the role of varicocele in male infertility.[J].2012,2012.
- [3]Xue-Sen, Zhang,Yah-He, Lue,Shu-Hua, Guo,etc.Expression of HSP105 and HSP60 during germ cell apoptosis in the heat-treated testes of adult cynomolgus monkeys (macaca fascicularis).[J].2005,10.
- [4]Jones DP,Zhivotovsky B,Cai J,etc.Presence of a pre-apoptotic complex of pro-caspase-3, Hsp60 and Hsp10 in the mitochondrial fraction of jurkat cells.[J].1999,18(8).
- [5]W W, Lin,D J, Lamb,T M, Wheeler,etc.Apoptotic frequency is increased in spermatogenic maturation arrest and hypospermatogenic states.[J].1997,158(5).
- [6]Ricardo D.Moreno,Paulina Urriola-Muñoz,Raúl Lagos-Cabré.The emerging role of matrix metalloproteases of the ADAM family in male germ cell apoptosis[J].2011,1(3).
- [7]Livak KJ,Schmittgen TD.Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.[J].2001,25(4).
- [8]Lone Schmidt,Kirstine Münster,Peter Helm.Infertility and the seeking of infertility treatment in a representative population[J].1995,102(12).
- [9]A. Werner,A. Meinhardt,J. SeitzM. Bergmann.Distribution of heat-shock protein 60 immunoreactivity in testes of infertile men[J].1997,288(3).
- [10]Tanji N,Iseda T,Yokoyama M,etc.Expression of heat shock proteins in developing and degenerating rat testes.[J].1999,43(3).
- [11]Wang H,Sun Y,Wang L,etc.Hypoxia-induced apoptosis in the bilateral testes of rats with left-sided varicocele: a new way to think about the varicocele.[J].2010,31(3).
- [12]Turner TT.The study of varicocele through the use of animal models.[J].2001,7(1).