Abstract： Objective To screen the oligopeptide with specific affinity to C-terminal activating region 2 (CTAR2) of EB virus latent membrane protein 1 (LMP1) by using the Ph D-12 Phage Display Peptide Library kit.Methods Purified CTAR2 protein was used to screen oligopeptide from Ph D -12Phage Display Peptide Library.Affinity of phage clones to CTAR2 was identified by sandwich ELISA.Dot blot assay was applied for identifying positive clones.DNA sequencing and analysis for these positive clones were performed.Results Yield of oligopeptide screened by CTAR2 protein in Round Ⅲ was 143 times of Round I [ (3.0× 10-6)/(2.1 × 10-8) ],with satisfactory enrichment of phage clones.All the screened clones efficiently bound to CTAR2 as shown by sandwich ELISA,and the positive rate of binding was 100％ as confirmed by Dot blot assay.A common sequence of the oligopeptide on phages (GLKHHSPGLLLY) was detected by DNA sequencing.Conclusion A oligopeptide sequence GLKHHSPGLLLY binding to CTAR2 of EBV LMP1 is screened,which provides a laboratory evidence for further research on the role of LMP1 in oncogenesis and for development of small molecule vaccines against LMP 1.