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In vio osteogenic potential of an implanted complex containing hBMP7-modified BMSCs and biomimetic composite scaffolds

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Author:
No author available
Journal Title:
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
Issue:
5
DOI:
10.3760/cma.j.issn.1674-1927.2010.05.006
Key Word:
骨形态发生蛋白质类;间质干细胞;骨替代材料;组织工程;Bone morphogenetic proteins;Mesenchymal stem cells;Bone replacement materials;Tissue engineering

Abstract: Objective To investigate the osteogenic potential in vivo of a cultured complex containing human bone morphogentic protein(hBMP)7-modified rabbit bone marrow mesenchymal stem cells (BMSCs) and bioglass-collagen-hyaluronic acid-phosphatidylserine (BG-COL-HYA-PS), a biomimetic composite scaffolds, implanted in segmental radial defect in rabbit models. Methods Rabbit BMSCs were cultured and transfected with rAAV2-hBMP7 or rAAV2-EGFP plasmids respectively in vitro. The transfected or non-transfected BMSCs were then cultured with biomimetic BG-COL-HYA-PS scaffolds for 7 days, and implanted into the radial segmental defect in 3 groups of rabbits (n=6 each). Eight and 12 weeks after implantation, gross inspection, imaging and histological studies were performed to observe the repair of bone defect. The biomechanics of repaired tissues in each group as compared with normal controls (n=3) was determined on week 12. Results Good biocompatibility, strong osteogenic potential and bone repairing was found for the complex of BG-COL-HYA-PS scaffolds and rAAV2-hBMP-7 transfected BMSCs after implanted in radial segmental defect of rabbit models. However, the strength of newly-formed rabbit radius was weaker than normal radius in terms of maximum pressure load [ (188.46± 12.24)N vs (203.25± 19.29)N, P<0.05 ].Conclusion A gene modified tissue-engineering bone, constructed by BMSCs transfected with hBMP-7 gene and biomimetic BG- COL-HYA- PS composite scaffolds, shows better capacity for bone repairing.However, the newly- formed bone tissue appears biomechanically insufficient during the early phase compared with normal bone tissue.

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