Abstract： Objective To evaluate the efficiency of poly hydroxybutyrate-hydroxyvalerate (PHBV) membrane and PH BV - sol - gel bioactive glass (PHBV - SGBG) composite for promoting bone regeneration.methods Canine tibial defect models were established and divided into four groups: PHBV membrane +PHBV-SGBG group, PHBV membrane group, PHBV-SGBG group and control group. Specimens were respectively collected from each group at 2, 4, 8 and 12 weeks after operation for bone growth assessment over the bone defect region by scanning electron microscopy. Quantitative determination of Ca and P over micro-areas of newly formed bone was also performed. Results New-bone formation in varying degrees was noted in all the treatment groups (PHBV membrane + PHBV-SGBG group, PHBV membrane group and PHBV-SGBG group). Repair and reconstruction over bone defect area was basically completed 12 weeks after operation in PHBV membrane + PHBV-SGBG group, with no statistical significance between Ca/P ratios of newly formed bone (1.625±0.037) and surrounding normal bone (1.625±0.063) (P＞0.05). Newly formed bone in the control group appeared porous, indicating a relatively high energy spectrum of organic tissues.The discrepancy between PHBV-SGBG group and PHBV membrane group had no statistical significance although PHBV-SGBG group seemed to have better bone repair (P＞0.05). Conclusion PHBV membrane induces bone regeneration, while PHBV-SGBG composite results in good bone conductivity and formation.Combined use of PHBV membrane and PHBV-SGBG composite shows a synergistic effect of repair of bone defects.