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Change of myocardial alpha B-crystallin after different kinds of preconditioning against ischemia/reperfusion injury in rats

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Author:
No author available
Journal Title:
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
Issue:
5
DOI:
10.3760/cma.j.issn.1674-1927.2009.05.001
Key Word:
远程预处理;缺血预处理;腺苷;缺血再灌注损伤;αB品状体蛋白;Remote preconditioning;Ischemic preconditoning;Adenosine;Ischemia reperfusion injury;αB-crystallin

Abstract: Objective To evaluate the cardioprotective effects of three different preconditioning methods and the change of myocardial α B-crystallin after these preconditioning methods against ischemia /reperfusion injury in rats. Methods Twenty-four SD rats were randomly divided into 4 groups (n=6 each): ischemia reperfusion group (IR), ischemia preconditioning group (IP), adenosine preconditioning group (AP) and remote preconditioning group (RP). The ischemia/reperfusion rat models were established in vivo. Hemodynamics of heart function before and after ischemia/reperfusion was recorded. At the end of reperfusion, serum cardiac troponin T (cTnT), malondialdehyde (MDA) , superoxide dismutase (SOD) and expression of α B-crystallin in myocardium were detected. Results At 120 minutes after reperfusion, significant increase in ±dp/dt_(max), was recorded in rats of group IP, AP and RP as compared with those of group IR (all P<0.05). Lower level of serum cTnT [(12.898±2.887), (5.049±4.387), (7.049±4.387) μg/L vs (22.902±3.146) μg/L, all P<0.05] was found in group IP, AP and RP as compared to group IR, so was the level of MDA [ (10.648±3.635), (11.736±8.903), (9.834±6.128) μmol/L vs (16.083±10.423) μmol/L,all P<0.05] ; but the level of SOD was just the reverse [(82.808±22.407), (162.266±54.128), (102.266± 34.134) U/ml vs (76.757±39.446) U/ml, all P<0.05]. Expression of αB-crystallin in these three groups was significantly higher than that in IR group (all P<0.05). AP group had a higher SOD level compared with IP and RP groups and a lower level of cTnT compared with IP group (all P<0.05). Conclusion Remote preconditioning and adenosine preconditioning may mimic the protective effects of ischemic preconditioning. Preconditioning attenuates myocardial isehemia/reperfusion injury in vivo possibly through up-regulation of αB-crystallin expression in rats.

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