Abstract： Objective To prepare neotype sodium alginate tissue engineering scaffold stuff(stuff for short) with ethylene diamine as cross linking agent and EDC as catalyzer, and to examine the cell compatibility. Methods Human fibroblasts were cultured in vitro, and the effect of the stuff impregnating solution on cell proliferation was evaluated by methyl thiazolyl tetrazolium (MTT) method. Cell growth situation in stuff impregnating solution was observed by microscopy. Human fibroblasts suspension were seeded on the surface of stuff to prepare human fibroblasts and the stuff complex. After 7 days, the cells adhesion to the complex surface and their growth status were observed by scanning electron microscopy, and cell compatibility of the stuff was evaluated. Results After 1,2,4,7 days in stuff impregnating solution, the relative growth rates (RGR) were 98.00%, 104.10%, 110.80%, 93.17% respectively and toxicities to human fibroblasts of the stuff were all 0 or 1 grade. Human fibroblasts grew well in stuff impregnating solution under invert microscope. Human fibroblasts had good adhesion to the stuff on the complex surface after 7 days in scanning electron microscopy, and the cells on the stuff surface grew well to form many pseudopodia and secreted cell matrix. Conclusion The neotype alginate tissue engineering scaffold stuff has good cell compatibility with the human fibroblasts, which can provide cell growth supplement and experimental evidence for further medical application.