Paper - WANFANG MED ONLINE

You Position: Home > Paper

芯片实验室在细胞研究中的应用进展

( views:244, downloads:112 )

Author:: No author available

Journal Title:: CHINESE JOURNAL OF BIOMEDICAL ENGINEERING

Issue:: 6

DOI:: 10.3760/cma.j.issn.1674-1927.2008.06.019

Key Word:: No keyword available

Abstract： @@ 微流控芯片或称微全分析系统(miniaturized total analysis system,μ-TAS)、芯片实验室一般是指把生物和化学等领域中所涉及的样品制备、生物与化学反应、分离检测等基本操作单位集成或基本集成在一块几平方厘米的芯片上,用以完成不同的生物或化学反应过程(包括细胞培养),并对其产物进行分析的一种技术[1].

Reference

[1]林炳承,秦建华.微流控芯片实验室[J].2005,(5).
[2]Bontoux N,Dauphinot L,Vitalis T,etc.Integrating whole transcriptome assays on a lab-on-a-chip for single cell gene profiling[J].2008,8(3).
[3]Paul Hung,Luke P.Lee,Yolanda Zhang,etc.On-chip cell lysis by local hydroxide generation[J].2005,5(2).
[4]Alona Muzikansky,Sunitha Nagrath,Daphne W. Bell,etc.Isolation of rare circulating tumour cells in cancer patients by microchip technology[J].2007,450(7173).
[5]Hung PJ,Lee LP,Rao VM,etc.Nanoliter scale microbioreactor array for quantitative cell biology[J].2006,94(1).
[6]Eric Leclerc,Yasuyuki Sakai,Teruo Fujii.Cell Culture in 3-Dimensional Microfluidic Structure of PDMS (polydimethylsiloxane)[J].2003,5(2).
[7]Xue-Feng Yin,Zhao-Lun Fang,Jian Gao.Integration of single cell injection, cell lysis, separation and detection of intracellular constituents on a microfluidic chip[J].2004,4(1).
[8]Sinclair J.,Karlsson M.,Olofsson J.,etc.A cell-based bar code reader for high-throughput screening of ion channel-ligand interactions[J].2002,74(24).
[9]P. B. Monaghan,H. Sedgwick,F. Caron.Lab-on-a-chip technologies for proteomic analysis from isolated cells[J].2008,5(Suppl.2).
[10]Arnold FH.,Quake SR.,Spence C.,etc.An integrated microfabricated cell sorter[J].2002,74(11).
[11]Liu X,Ye N,Lin B,etc.Characterizing doxorubicin-induced apoptosis in HepG2 cells using an integrated microfluidic device.[J].2007,28(7).
[12]Li CW.,Yang J.,Yang MS..Cell docking and on-chip monitoring of cellular reactions with a controlled concentration gradient on a microfluidic device[J].2002,74(16).
[13]Anna Tourovskaia,Albert Folch,Xavier Figueroa-Masot.Differentiation-on-a-chip:A microfluidic platform for long-term cell culture studies[J].2005,5(1).
[14]Luke P.Lee,Dino Di Carlo,Ki-Hun Jeong.Reagentless mechanical cell lysis by nanoscale barbs in microchannels for sample preparation[J].2003,3(4).
[15]Ana Valero,Francisco Merino,Albert Van den Berg,etc.Apoptotic cell death dynamics of HL60 cells studied using a microfluidic cell trap device[J].2005,5(1).
[16]M R, Wilkins,C, Pasquali,R D, Appel,etc.From proteins to proteomes: large scale protein identification by two-dimensional electrophoresis and amino acid analysis.[J].1996,14.
[17]HOSSEIN SALIMI-MOOSAVI,RICHARD SMITH,D. JED HARRISON,etc.β-Galactosidase Assays of Single-Cell Lysates on a Microchip: A Complementary Method for Enzymatic Analysis of Single Cells[J].2004,92(1).
[18]Jeon NL,Lee AP,Monuki ES,etc.Unique dielectric properties distinguish stem cells and their differentiated progeny.[J].2008,26(3).
[19]XiuJun Li,Victor Ling,Paul C.H.Li.Same-Single-Cell Analysis for the Study of Drug Efflux Modulation of Multidrug Resistant Cells Using a Microfluidic Chip[J].2008,80(11).
[20]Jerry Lii,Wern-Jir Hsu,Hesam Parsa.Real-Time Microfluidic System for Studying Mammalian Cells in 3D Microenvironments[J].2008,80(10).
[21]Arto Heiskanen,Christer Spegel,Simon Pedersen.Fully automated microchip system for the detection of quantal exocytosis from single and small ensembles of cells[J].2008,8(2).
[22]Nevill JT,Cooper R,Dueck M,etc.Integrated microfluidic cell culture and lysis on a chip[J].2007,7(12).
[23]Sorger PK,Jensen KF,El-Ali J.Cells on chips[J].2006,442(7101).
[24]van den Berg A,Andersson H,Emmelkamp J,etc.The potential of autofluorescence for the detection of single living cells for label-free cell sorting in microfluidic systems.[J].2004,25(21).
[25]Fox MB,van den Berg A,Mastwijk HC,etc.Electroporation of cells in microfluidic devices: a review[J].2006,385(3).
[26]Leach AM.,Daridon A.,Zare RN.,etc.Microfluidic device for single-cell analysis[J].2003,75(14).
[27]Zare RN,Wu H,Wheeler A.Chemical cytometry on a picoliter-scale integrated microfluidic chip.[J].2004,101(35).
[28]Fumihito Arai,Hisataka Maruyama,Akihiko Ichikawa,etc.On chip single-cell separation and immobilization using optical tweezers and thermosensitive hydrogel[J].2005,5(12).
[29]Viviani A,Delamarche E,Lovchik R,etc.Cellular microarrays for use with capillary-driven microfluidics[J].2008,390(3).
[30]Wang S,Wan Y,Bei J,etc.Adhesion and proliferation of OCT-1 osteoblast-like cells on micro- and nano-scale topography structured poly(l-lactide).[J].2005,26(21).
[31]Paul C. H. Li,Xiujun Li.Microfluidic Selection and Retention of a Single Cardiac Myocyte, On-Chip Dye Loading, Cell Contraction by Chemical Stimulation, and Quantitative Fluorescent Analysis of Intracellular Calcium[J].2005,77(14).
[32]Sun Y,Fang ZL,Yin XF,etc.Determination of reactive oxygen species in single human erythrocytes using microfluidic chip electrophoresis[J].2005,382(7).
[33]Lin BC,Ye NN,Liu X,etc.Microfluidic devices for the analysis of apoptosis[J].2005,26(19).
[34]Sato K,Kitamori T,Goto M,etc.Micro- and nanometer-scale patterned surface in a microchannel for cell culture in microfluidic devices[J].2008,390(3).