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Evaluation of thymic recent output function in patients with ANLL by quantitative analysis of TRECs content
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- Author:
- No author available
- Journal Title:
- JOURNAL OF MODERN CLINICAL MEDICAL BIOENGINEERING
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1008-634X.2006.04.011
- Key Word:
- T细胞受体重排删除DNA环;胸腺近期输出功能;白血病;实时定量PCR
Abstract： 目的 检测急性非淋巴细胞白血病(ANLL)患者 T细胞受体重排删除DNA环(signal joint T-cell receptor excision DNA circles sjTRECs,TRECs)的含量,从而探讨患者的初始型naive T细胞水平和胸腺近期输出功能特点.方法利用实时定量PCR(TaqMan)方法,检测77例ANLL患者外周血单个核细胞(PBMC)TRECs的水平,并根据外周血中CD3阳性率计算CD3细胞中TRECs水平.14例ANLL缓解期患者和14例正常人外周血作为对照.结果 ANLL患者外周血中TRECs含量为(0.43±0.92)拷贝/1 000 PBMCs, (2.02±3.25)拷贝/1 000 CD3+细胞,明显低于正常人TRECs水平[(4.10±3.65)拷贝/1 000 PBMCs和(6.84±4.71)拷贝/1 000 CD3+细胞,P=0.0000和P=0.0001]和缓解期患者[(2.19±2.49)拷贝/1 000 PBMCs和(6.30±7.13)拷贝/1 000 CD3+细胞,P=0.0000和P=0.0005].各亚型ANLL患者的TREC水平差异无统计学意义(P＞0.05).结论 绝大多数ANLL型患者胸腺近期输出naive T细胞功能明显降低,缓解期患者的胸腺近期输出功能有一定程度恢复.
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