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Study on the Distribution of the Polymorphisms of Apolipoprotein E in Pati ents with Atherosclerotic Cerebral Infarction with PCR-RFLP
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- Author:
- No author available
- Journal Title:
- JOURNAL OF MODERN CLINICAL MEDICAL BIOENGINEERING
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1674-1927.2000.04.004
- Key Word:
- 载脂蛋白E;基因多态性;聚合酶链反应-限制性片段长度多;态性;动脉硬化性脑梗死
Abstract： 目的研究动脉硬化性脑梗死(ACI)患者中的载脂蛋白E(ApoE)基因的分布.方法利用聚合酶链式反应(PCR)技术扩增ApoE基因含编码112位和158位氨基酸的片段,并用限制性片段长度多态性(RFLP)技术对ACI患者和相应健康对照的ApoE基因进行分型,从而进行ACI与ApoE等位基因多态性的关联分析.结果ACI患者中ApoE等位基因ε4占28.30%,明显高于正常人对照组的7.64%,而等位基因ε3则占57.55%,低于正常人对照组的84.12%,均有极显著性差异(p<0.01).结论PCR-RFLP是一种快速有效的ApoE基因分型方法,ε4可能为ACI的易感因子,而ε3则为保护因子.
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