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Dual regulation of Qizhiweitong particles on gastric motility in the model rats with gastroparesis
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- Author:
- No author available
- Journal Title:
- Acta Physiologica Sinica
- Issue:
- 5
- DOI:
- 10.13294/j.aps.2022.0082
- Key Word:
- 气滞胃痛颗粒;胃轻瘫;胃动力;胃肌电;胃轻瘫模型大鼠
Abstract： 本文旨在研究气滞胃痛颗粒(Qizhiweitong particles,QZWT)对胃轻瘫模型大鼠胃动力的影响,为其临床治疗提供理论与实验依据.选用SPF级雄性Sprague-Dawley(SD)大鼠,双侧中脑黑质注射6-羟基多巴胺建立胃轻瘫大鼠模型,单次胃饲1、10、30、100、200、450或675 mg/kg QZWT或连续7天每天胃饲675 mg/kg QZWT,用胃固体食物排空实验和在体X线成像技术检测胃排空率,用PowerLab生物信号采集系统检测离体和在体胃体纵行肌收缩,用植入式生理信号无线遥测系统检测胃肌电活动,用蛋白免疫印迹检测胃肌层促炎蛋白酶表达水平.结果显示,QZWT单次给药可剂量依赖性地抑制正常大鼠离体胃平滑肌的收缩活动.QZWT单次给药抑制对照和模型组大鼠在体胃动力及胃肌电活动.与对照组相比,模型组大鼠的胃排空率、在体和离体胃动力以及胃肌电活动均显著降低,QZWT连续给药可改善模型组大鼠上述胃动力指标.QZWT单次给药抑制离体胃肌条收缩,阿托品和一氧化氮合酶抑制剂预处理均对此作用无影响.QZWT连续给药可下调模型组大鼠升高了的一氧化氮合酶和环氧合酶2的蛋白表达水平.上述结果提示,临床上QZWT缓解胃痛的作用可能与其抑制胃动力的即刻作用有关,与乙酰胆碱和一氧化氮通路无关.QZWT长期治疗可增强胃肌电活动、促进胃平滑肌收缩和胃排空,从而改善胃动力,其作用机制可能部分与炎症抑制有关.
- [1]蒋福宇,邵平,韩凌,等.气滞胃痛颗粒药理作用及临床应用研究进展[J].2021,(15).
- [2]郝庆,李岩.不同品种柴胡枳实促胃肠动力作用及机制探讨[J].2002,(3).
- [3]姚元生,郑丽飞,朱进霞.采用植入式生理信号无线遥测系统记录清醒大鼠自由活动状态下胃的肌电信号[J].2017,(2).
- [4]Drossman, Douglas A..Functional Gastrointestinal Disorders: History, Pathophysiology, Clinical Features, and Rome IV[J].2016,150(6).
- [5]李爽,包永睿,王帅,等.基于多功效多指标药效综合评价的气滞胃痛颗粒组分配伍研究[J].2014,(24).
- [6]Koloski, N. A.,Jones, M.,Talley, N. J..Evidence that independent gut-to-brain and brain-to-gut pathways operate in the irritable bowel syndrome and functional dyspepsia: a 1-year population-based prospective study[J].2016,44(6).
- [7]Song,J.,Zheng,L.,Zhang,X.,etc.Upregulation of β1-adrenoceptors is involved in the formation of gastric dysmotility in the 6-hydroxydopamine rat model of Parkinson's disease[J].2014,164(1).
- [8]包永睿,王帅,孟宪生,等.气滞胃痛颗粒促胃肠动力作用谱效关系网络模型的构建[J].2014,(5).
- [9]Li XF,Feng XY,Tang YY,etc.Reduced expression of choline acetyltransferase in vagal motoneurons and gastric motor dysfunction in a 6-OHDA rat model of Parkinson's disease.[J].2011,1420.
- [10]Bonney Reed-Knight,Robyn Lewis Claar,Jennifer Verrill Schurman,etc.Implementing psychological therapies for functional GI disorders in children and adults[J].2016,10(9).
- [11]Xiu Xiao-Lin,Zheng Li-Fei,Liu Xiao-Yu,etc.Gastric smooth muscle cells manifest an abnormal phenotype in Parkinson’s disease rats with gastric dysmotility[J].2020,381(2).
- [12]杨晓娟,胡黄婉茵,张海珠,等.基于网络药理学的气滞胃痛颗粒抗炎镇痛活性作用分析[J].2019,(13).
- [13]郝庆,李岩,尹洪涛.不同品种柴胡枳实对小鼠胃肠动力作用的比较研究[J].1998,(S2).
- [14]姚东,孟宪生,王帅,等.气滞胃痛颗粒全时段多波长融合指纹图谱研究及多成分定量分析[J].2013,(10).
- [15]Natasha A, Koloski,Nicholas J, Talley,Philip M, Boyce.Epidemiology and health care seeking in the functional GI disorders: a population-based study.[J].2002,97(9).
- [16]Lam, Jennifer Y.,Kidane, Biniam,Manji, Farouq,etc.Improved Health-Related Quality of Life After Surgical Management of Severe Refractory Constipation-Dominant Irritable Bowel Syndrome[J].2015,100(1).
- [17]Zheng,L.-F.,Song,J.,Fan,R.-F.,etc.The role of the vagal pathway and gastric dopamine in the gastroparesis of rats after a 6-hydroxydopamine microinjection in the substantia nigra[J].2014,211(2).
- [18]Chang, Xin,Wang, Shuai,Bao, Yong-rui,etc.Multicomponent, multitarget integrated adjustment - Metabolomics study of Qizhiweitong particles curing gastrointestinal motility disorders in mice induced by atropine[J].2016,189.
- [19]Qing Su,Sheng-Liang Chen,Hua-Hong Wang,etc.A Randomized, Double-Blind, Multicenter, Placebo-Controlled Trial of Qi-Zhi-Wei-Tong Granules on Postprandial Distress Syndrome-Predominant Functional Dyspepsia[J].2018,131(13).
- [20]Xiaoli, Zhang,Yun, Li,Chenzhe, Liu,etc.Alteration of enteric monoamines with monoamine receptors and colonic dysmotility in 6-hydroxydopamine-induced Parkinson's disease rats.[J].2015,166(2).