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17β-estradiol inhibits interleukin-lβ-induced rat nucleus pulposus cell apoptosis through the PI3K/Akt/mTOR signal pathway
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- Author:
- No author available
- Journal Title:
- Acta Physiologica Sinica
- Issue:
- 1
- DOI:
- 10.13294/j.aps.2021.0002
- Key Word:
- 17β-雌二醇;髓核细胞;mTOR;白介素lβ;凋亡
Abstract： 髓核细胞(nucleus pulposus cells,NPCs)的异常凋亡是导致椎间盘退变(intervertebral disc degeneration,IVDD)的主要原因.本研究组前期研究显示,17β-雌二醇(17β-estradiol,E2)能够通过P13K/Akt信号通路抑制白介素lβ(interleukin-1β,IL-lβ)诱导的大鼠椎间盘NPCs凋亡.本研究旨在探讨PI3K/Akt途径的下游蛋白是否参与E2对NPCs凋亡的抑制作用.用胰蛋白酶消化法分离原代大鼠NPCs,采用E2和PI3K/Akt信号通路下游蛋白的不同抑制剂预处理后用IL-lβ处理,用Annexin V/PI染色法检测凋亡率,用CCK-8法检测细胞活力,用细胞黏附试验检测NPCs与II型胶原的黏附能力,用Western blot检测哺乳动物雷帕霉素靶蛋白(mammalian target of Rapamycin,mTOR)、糖原合成酶激酶-3β(glycogen synthase kinase-3β,GSK-3β)和核因子κB(nuclear factor kappaB,NF-κB)磷酸化水平.结果显示,E2显著抑制IL-lβ诱导的NPCs凋亡,逆转由IL-1β引起的细胞活力和黏附能力的降低,抑制IL-lβ对mTOR磷酸化水平的下调作用,而雷帕霉素可以阻断E2的这些保护作用.以上结果提示,E2可能通过PI3K/Akt/mTOR信号通路抑制IL-1β诱导的NPCs凋亡.
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