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FOXO4 maintains senescence in human umbilical cord mesenchymal stem cells by repressing apoptosis

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Author:
No author available
Journal Title:
Acta Physiologica Sinica
Issue:
4
DOI:
10.13294/j.aps.2020.0045
Key Word:
人脐带间充质干细胞;叉头盒O4;细胞衰老;凋亡

Abstract: 本文旨在探讨叉头盒O4 (forkhead box O4,FOXO4)在人脐带间充质干细胞(human umbilical cord-derived mesenchymal stem cells, hUC-MSCs)衰老中的作用.采用自然传代法诱导hUC-MSCs衰老,用慢病毒shRNA抑制FOXO4表达,用β-半乳糖苷酶染色法检测细胞衰老情况,用CCK-8法检测细胞活性,用流式细胞术检测细胞凋亡,用qPCR和Western blot检测细胞Bcl-2、Bax、FOXO4、白介素6(interleukin 6,IL-6)和cleaved Caspase-3的表达情况,用免疫荧光染色法检测细胞FOXO4表达情况,用ELISA检测细胞IL-6分泌量.结果 显示,相比第一代hUC-MSCs,衰老hUC-MSCs中FOXO4和Bax表达水平上调,Bcl-2和cleaved Caspase-3表达水平下调,IL-6 mRNA表达水平上调、分泌量增加.抑制FOXO4的表达后,衰老hUC-MSCs凋亡增加,细胞活力下降,IL-6 mRNA表达水平下调,分泌量降低.上述结果提示,FOXO4能通过抑制凋亡来维持衰老hUC-MSCs活力和功能,加速整个细胞集落衰老.

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