Abstract： Spinal α-motoneurons directly innervate skeletal muscles and function as the final common path for movement and behavior.The processes that determine the excitability of motoneurons are critical for the execution of motor behavior.In fact,it has been noted that spinal motoneurons receive various neuromodulatory inputs,especially monoaminergic one.However,the roles of histamine and hypothalamic histaminergic innervation on spinal motoneurons and the underlying ionic mechanisms are still largely unknown.In the present study,by using the method of intracellular recording on rat spinal slices,we found that activation of either H1 or H2 receptor potentiated repetitive firing behavior and increased the excitability of spinal α-motoneurons.Both of blockage of K+ channels and activation ofNa+-Ca2+ exchangers were involved in the H1 receptor-mediated excitation on spinal motoneurons,whereas the hyperpolarization-activated cyclic nucleotide-gated (HCN) channels were responsible for the H2 receptor-mediated excitation.The results suggest that,through switching functional status of ion channels and exchangers coupled to histamine receptors,histamine effectively biases the excitability of the spinal α-motoneurons.In this way,the hypothalamospinal histaminergic innervation may directly modulate final motor outputs and actively regulate spinal motor reflexes and motor execution.