Paper - WANFANG MED ONLINE

You Position: Home > Paper

Anti-injury effect of hydrogen-enriched water in a rat model of liver injury induced by aflatoxin B1

( views:29, downloads:0 )

Author:: No author available

Journal Title:: Acta Physiologica Sinica

Issue:: 5

DOI:: 10.13294/j.aps.2019.0058

Key Word:: 富氢水;黄曲霉毒素B1;急性肝损伤;抗氧化;氧化应激

Abstract：本文旨在研究富氢水在黄曲霉毒素B1(aflatoxin B1,AFB1)致大鼠急性肝损伤模型中的抗损伤作用及保护机制.将健康雄性Sprague-Dawley (SD)大鼠随机分为对照组、模型组(AFB1组)和富氢水处理组(AFB1+H2组),采用单次灌胃AFB1 (2.0 mg/kg)建立大鼠急性AFB1肝损伤模型,并给予富氢水灌胃干预.用HE染色观察肝组织病理变化,下腔静脉取血检测血清肝功能指标,取肝组织检测丙二醛(malonaldehyde,MDA)和还原型谷胱甘肽(glutathione,GSH)含量,用Westem blot检测MAPK信号通路蛋白(ERK、JNK和p38 MAPK)磷酸化水平.结果显示,相对AFB1组,AFB1+H2组大鼠体重增加,AFB1引起的急性肝损伤显著减轻,血清谷丙转氨酶、谷草转氨酶活性和总胆红素含量降低,肝组织中MDA含量降低,还原型GSH含量升高,肝组织ERK、JNK、p38 MAPK磷酸化水平显著下调.上述结果提示,富氢水可减轻AFB1肝损伤,其机制可能与富氢水减轻AFB1引起的氧化应激、抑制MAPK信号转导通路的激活有关.

Reference

[1]陈烨,王旭姣,杨博,等.富氢水对肢体缺血/再灌注大鼠肺损伤及脂肪因子chemerin的影响[J].2017,(3).
[2]曹倩,魏灵荣,鲁玲玲,等.星形胶质细胞通过谷胱甘肽保护MN9D细胞抵抗鱼藤酮所致氧化应激[J].2007,(3).
[3]王赞,刘莉,张喆,等.富氢水对心肌缺血再灌注模型大鼠心肌损伤的保护作用[J].2018,(8).
[4]孙伟龙,王思平,陈昊,等.富氢水对乙醇诱导急性胃损伤的保护作用及其机制[J].2018,(1).
[5]杨培,冷波,李波,等.富氢水对重症急性胰腺炎大鼠血清炎症细胞因子及肺组织氧化应激反应的影响[J].2015,(18).
[6]陈建民,张雪辉,杨美华,等.黄曲霉毒素检测方法研究进展[J].2005,(24).
[7]耿欣,刘彦芳,高玉梅,等.β- HCG、ERK1/2及 MMP -2在卵巢癌中的表达及意义[J].2015,(9).
[8]姜勇,龚小卫.MAPK信号转导通路对炎症反应的调控[J].2000,(4).
[9]章丽霞,刘钧,黄一凡,等.ERK2和p-ERK1/2蛋白在宫颈癌中的表达和意义[J].2012,(2).
[10]Zhan J,McCurry KR,Cardinal JS,etc.Oral hydrogen water prevents chronic allograft nephropathy in rats.[J].2010,77(2).
[11]L, Cai,Y J, Kang.Oxidative stress and diabetic cardiomyopathy: a brief review.[J].2001,1(3).
[12]Aoki H,Kang PM,Hampe J,etc.Direct activation of mitochondrial apoptosis machinery by c-Jun N-terminal kinase in adult cardiac myocytes.[J].2002,277(12).
[13]Ishikawa M,Ohta S,Fukuda K,etc.Inhalation of hydrogen gas suppresses hepatic injury caused by ischemia/reperfusion through reducing oxidative stress.[J].2007,361(3).
[14]赵雅娟,殷飞.ERK-1与Cyclin D1在肝细胞肝癌组织中的表达[J].2010,(2).
[15]赖丹,黄非.过氧化氢对老年豚鼠耳蜗外毛细胞大电导钙激活钾通道电流的影响[J].2014,(3).
[16]Sun,Liu C,Cui J.Hydrogen therapy may be an effective and specific novel treatment for acute radiation syndrome.[J].2010,74(1).
[17]艾青,葛璞,代洁,等.过氧化氢酶抑制剂氨基三唑减轻急性酒精性肝损伤[J].2015,(1).
[18]Ohsawa I,Watanabe M,Ishikawa M,etc.Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals.[J].2007,13(6).
[19]Ghneim, Hazem K.,Alshebly, Mashael M..Biochemical Markers of Oxidative Stress in Saudi Women with Recurrent Miscarriage[J].2016,31(1).
[20]李映慧,刘少波,张皓云,等.雷公藤红素对抗过氧化氢诱导肌萎缩性侧索硬化症细胞模型氧化损伤的保护作用[J].2017,(6).