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Dopamine D2 receptor may be involved in the regulation of cortical-striatum synaptic transmission and autonomic activity in PD mice by exercise
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- Author:
- No author available
- Journal Title:
- Acta Physiologica Sinica
- Issue:
- 4
- DOI:
- 10.13294/j.aps.2019.0029
- Key Word:
- 帕金森病;运动干预;皮层-纹状体通路;多巴胺Ⅱ型受体;自主活动能力;突触传递
Abstract： 本研究旨在揭示皮层-纹状体突触后膜多巴胺D2型受体(D2 receptor,D2R)在运动改善帕金森病(Parkinson's disease,PD)小鼠行为功能障碍中的作用.选取C57/BL6雄性成年小鼠,随机分为对照组、PD组和PD运动组.纹状体两点注射6-羟多巴胺(6-hydroxydopamine,6-OHDA)建立单侧损伤PD模型.运动干预方案为匀速跑台训练(16 m/min,40 min/d,每周5d,共持续4周).采用旷场实验评价小鼠自主活动能力,用离体脑片记录兴奋性突触后场电位(field excitatory postsynaptic potential,fEPSP)来评价皮层-纹状体突触传递效能,并观察D2R激动剂干预对小鼠自主活动能力和皮层-纹状体突触传递的影响.结果 显示,和PD组相比,PD运动组小鼠自主运动距离和快速移动占比显著增加(P＜0.05),递增刺激强度(0.75～3.00 pA)下fEPSP最大幅值显著降低(P＜0.05),刺激-反应曲线斜率降低.和未给予D2R激动剂的PD小鼠相比,D2R激动剂处理的PD小鼠运动距离及快速移动占比显著增加(P＜0.05),fEPSP峰值(P＜0.05)和刺激-反应曲线斜率降低.以上结果表明,早期运动干预或D2R敏动剂干预均可抑制PD小鼠皮层-纹状体突触传递效能异常增高的现象,并改善小鼠自主运动能力,提示皮层-纹状体突触后膜D2R可能是运动改善PD小鼠自主活动能力的重要细胞分子作用靶点.
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