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Vasodilation of quercetin on rat renal artery and the relationship with L-type voltage-gated Ca2+ channels and protein kinase C

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Journal Title:
Acta Physiologica Sinica
Key Word:
槲皮素;肾动脉;舒张;L-型电压依赖性钙通道;蛋白激酶C;quercetin;renal artery;relaxation;L-type voltage-gated Ca2+ channels;protein kinase C

Abstract´╝Ü To investigate the diastolic function of quercetin on rat renal artery in vitro and its mechanism,the tension of rat renal artery was recorded by multi myograph system,and the L-type voltage-gated Ca2+ channels (LVGC) current was recorded by whole-cell patch clamp technique.Quercetin produced relaxation effect on rat renal artery pre-contracted by 60 mmol/L KC1 or 1 × 10-5 mol/L phenylephrine,and the maximal diastolic percentage was (84.53 ± 7.35)% or (76.42 + 4.63)%.There was no statistical difference in the maximal diastolic percentage between endothelium-intact and endothelium-denuded groups.Pre-incubation of protein kinase C (PKC) inhibitor C6303 inhibited the maximal diastolic amplitude induced by quercetin.The peak current density of LVGC in rat renal artery vascular smooth muscle cells (VSMCs) was (23.17 ± 1.33) pA/pF.Quercetin (10 μmol/L) inhibited the peak current to (10.46 + 1.35) pA/pF,and the inhibition percentage was 54.86%.C6303 (1 μmol/L) partially reversed the inhibitory effect of quercetin,and the inhibition percentage was 62.08% (P < 0.05).These results suggest that quercetin can relax rat renal artery in vitro in a concentration-dependent and endotheliumindependent manner.The vasodilation of quercetin may be related to inhibition of LVGC current and activation of PKC.

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