Vasodilation of quercetin on rat renal artery and the relationship with L-type voltage-gated Ca2+ channels and protein kinase C
- Author:
- No author available
- Journal Title:
- Acta Physiologica Sinica
- Issue:
- 6
- DOI:
- 10.13294/j.aps.2017.0079
- Key Word:
- 槲皮素;肾动脉;舒张;L-型电压依赖性钙通道;蛋白激酶C;quercetin;renal artery;relaxation;L-type voltage-gated Ca2+ channels;protein kinase C
Abstract： To investigate the diastolic function of quercetin on rat renal artery in vitro and its mechanism,the tension of rat renal artery was recorded by multi myograph system,and the L-type voltage-gated Ca2+ channels (LVGC) current was recorded by whole-cell patch clamp technique.Quercetin produced relaxation effect on rat renal artery pre-contracted by 60 mmol/L KC1 or 1 × 10-5 mol/L phenylephrine,and the maximal diastolic percentage was (84.53 ± 7.35)％ or (76.42 + 4.63)％.There was no statistical difference in the maximal diastolic percentage between endothelium-intact and endothelium-denuded groups.Pre-incubation of protein kinase C (PKC) inhibitor C6303 inhibited the maximal diastolic amplitude induced by quercetin.The peak current density of LVGC in rat renal artery vascular smooth muscle cells (VSMCs) was (23.17 ± 1.33) pA/pF.Quercetin (10 μmol/L) inhibited the peak current to (10.46 + 1.35) pA/pF,and the inhibition percentage was 54.86％.C6303 (1 μmol/L) partially reversed the inhibitory effect of quercetin,and the inhibition percentage was 62.08％ (P ＜ 0.05).These results suggest that quercetin can relax rat renal artery in vitro in a concentration-dependent and endotheliumindependent manner.The vasodilation of quercetin may be related to inhibition of LVGC current and activation of PKC.
- [1]M, Bertolissi,A, De Monte,F, Giordano.Comparison of intravenous nifedipine and sodium nitroprusside for treatment of acute hypertension after cardiac surgery.[J].1900,64(7-8).
- [2].Quercetin, Hyperin, and Chlorogenic Acid Improve Endothelial Function by Antioxidant, Antiinflammatory, and ACE Inhibitory Effects[J].2017,82(4).
- [3]Sánchez,M.-J.,Zamora-Ros,R.,MaríaHuerta,J.,etc.Dietary flavonoid and lignan intake and mortality in a Spanish cohort[J].2013,24(5).
- [4]Giovanni E, Mann,David J, Rowlands,Francois Y L, Li,etc.Activation of endothelial nitric oxide synthase by dietary isoflavones: role of NO in Nrf2-mediated antioxidant gene expression.[J].2007,75(2).
- [5]C K, Chen,C R, Pace-Asciak.Vasorelaxing activity of resveratrol and quercetin in isolated rat aorta.[J].1996,27.
- [6]Juan Tamargo,Angel L. Cogolludo,Francisco Perez-Vizcaino,etc.Endothelium-Independent Vasodilator Effects of the Flavonoid Quercetin and Its Methylated Metabolites in Rat Conductance and Resistance Arteries[J].2002,302(1).
- [7]Russo M,Spagnuolo C,Tedesco I,etc.The flavonoid quercetin in disease prevention and therapy: facts and fancies.[J].2012,83(1).
- [8]Lodi F,Santos Buelga C,Hughes DA,etc.Glucuronidated and sulfated metabolites of the flavonoid quercetin prevent endothelial dysfunction but lack direct vasorelaxant effects in rat aorta.[J].2009,204(1).
- [9]G, Casabona.Intracellular signal modulation: a pivotal role for protein kinase C.[J].1997,21.
- [10]Jalili T,Wu TC,David Symons J,etc.Quercetin-supplemented diets lower blood pressure and attenuate cardiac hypertrophy in rats with aortic constriction.[J].2006,47(4).
- [11]Hongwei, Li,Qiang, Chai,David D, Gutterman,etc.Elevated glucose impairs cAMP-mediated dilation by reducing Kv channel activity in rat small coronary smooth muscle cells.[J].2003,285(3).
- [12]Huishuang Zheng,Jun Liu,Chong Liu,etc.Calcium-sensing receptor activating phosphorylation of PKCδ translocation on mitochondria to induce cardiomyocyte apoptosis during ischemia/reperfusion[J].2011,358(1-2).
- [13]Perez Vizcaino F,Duarte J,Jimenez R,etc.Antihypertensive effects of the flavonoid quercetin.[J].2009,61(1).
- [14]Murat, Ikizler,Nilüfer, Erkasap,Sadettin, Dernek,etc.Dietary polyphenol quercetin protects rat hearts during reperfusion: enhanced antioxidant capacity with chronic treatment.[J].2007,7(4).
- [15]Tamargo J,Perez Vizcaino F,Zaragoza Arnaez F,etc.Cardiovascular effects of isorhamnetin and quercetin in isolated rat and porcine vascular smooth muscle and isolated rat atria.[J].2002,68(4).
- [16]Liu,C.-J.,Lin,J.-Y..Anti-inflammatory effects of phenolic extracts from strawberry and mulberry fruits on cytokine secretion profiles using mouse primary splenocytes and peritoneal macrophages[J].2013,16(2).
- [17]Hou,X.,Liu,Y.,Niu,L.,etc.Enhancement of voltage-gated K+ channels and depression of voltage-gated Ca2+ channels are involved in quercetin-induced vasorelaxation in rat coronary artery[J].2014,80(6).
- [18]Zhang MS,Xue WX,Niu LG,etc.Vasorelaxant effect of taurine is diminished by tetraethylammonium in rat isolated arteries.[J].2008,580(1).
- [19]Kline, Loren,Karpinski, Edward.Quercetin relaxes guinea pig gallbladder strips[J].2016,36(10).