Effects of tetramethylpyrazine on trigeminal neuralgia induced by chronic constriction injury of infraorbital nerve in rats
- Author:
- No author available
- Journal Title:
- Acta Physiologica Sinica
- Issue:
- 1
- DOI:
- 10.13294/j.aps.2016.0097
- Key Word:
- 降钙素基因相关肽;三叉神经痛;川芎嗪;三叉神经节;calcitonin gene-related peptide;trigeminal neuralgia;tetramethylpyrazine;trigeminal ganglion
Abstract： 三叉神经痛(trigeminal neuralgia,TN)是局限于三叉神经1支或多支分布区域的反复发作的短暂剧烈疼痛,临床发病率高,严重影响患者生活质量,难以完全治愈.本研究旨在探讨川芎嗪(tetramethylpyrazine,TMP)对慢性压迫性损伤眶下神经(chronic constriction injury of the infraorbital nerve,ION-CCI)致大鼠TN的影响.Sprague-Dawley大鼠随机分为4组:假手术组(sham)、假手术+川芎嗪组(sham+TMP)、模型组(TN)、模型+川芎嗪组(TN+TMP).通过ION-CCI建立大鼠TN模型,术后2周每天一次腹腔注射川芎嗪(50 mg/kg).应用电子测痛仪观察各组大鼠机械痛敏阈值的变化.同时采用RT-PCR、蛋白印迹、免疫组织化学染色等方法检测大鼠术侧三叉神经节(trigeminal ganglia,TGs)中降钙素基因相关肽(calcitonin gene-related peptide,CGRP)的表达变化.术后15天内,TN组大鼠手术侧眶下神经面部感觉区域的机械痛敏阈值和sham组相比明显降低,术后第9天到第15天,TN+TMP组和TN组相比较阈值出现明显升高(P＜ 0.05).术后第15天,RT-PCR、蛋白印迹、免疫组织化学染色结果表明,与sham组相比,TN组大鼠TGs中CGRP表达明显升高(P＜0.05);与TN组相比,TN+TMP组大鼠TGs中CGRP表达显著降低(P＜0.05).以上结果表明,TGs中的CGRP对TN的痛觉传递有着重要作用,TMP对TN有一定的抑制作用,其机制可能和抑制TGs中CGRP表达有关.
- [1]Messlinger,K.,Lennerz,J.K.,Eberhardt,M.,etc.CGRP and NO in the trigeminal system: Mechanisms and role in headache generation[J].2012,52(9).
- [2]张彩娣,滕蕊,卢蓁,等.大鼠正畸牙移动过程中TRPV1和CGRP在三叉神经节中的表达变化[J].2015,(1).
- [3]Maria Adele Giamberardino,Giannapia Affaitati,Martina Curto,etc.Anti-CGRP monoclonal antibodies in migraine: current perspectives[J].2016,(8).
- [4]Lars Edvinsson,Janos Tajti,Bernadett Tuka,etc.Calcitonin gene-related peptide and its receptor components in the human sphenopalatine ganglion -- interaction with the sensory system.[J].2012,1435.
- [5]覃清霞,孔祥英,林娜.川芎嗪对神经发生影响的研究进展[J].2016,(1).
- [6]B P, Vos,A M, Strassman,R J, Maciewicz.Behavioral evidence of trigeminal neuropathic pain following chronic constriction injury to the rat's infraorbital nerve.[J].1994,14.
- [7]Vijayan, Gangadharan,Rohini, Kuner.Pain hypersensitivity mechanisms at a glance.[J].2013,6(4).
- [8]Rapoport, Alan M.,Bigal, Marcelo E.,Walter, Sarah.Therapeutic antibodies against CGRP or its receptor[J].2015,79(6).
- [9]Talisia Quallo,Clive Gentry,Stuart Bevan,etc.Activation of transient receptor potential ankyrin 1 induces CGRP release from spinal cord synaptosomes[J].2015,3(6).
- [10]Devesa, Isabel,Ferrandiz-Huertas, Clotilde,Mathivanan, Sakthikumar,etc.alpha CGRP is essential for algesic exocytotic mobilization of TRPV1 channels in peptidergic nociceptors[J].2014,111(51).
- [11]Gao Y,Xu C,Liang S,etc.Effect of tetramethylpyrazine on primary afferent transmission mediated by P2X3 receptor in neuropathic pain states.[J].2008,77(1).
- [12]Zakrzewska JM.Medical management of trigeminal neuropathic pains.[J].2010,11(8).