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Effect of enhanced green fluorescent protein fusion on Ano1 physiological feature
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- Author:
- No author available
- Journal Title:
- Acta Physiologica Sinica
- Issue:
- 6
- DOI:
- 10.13294/j.aps.2015.0077
- Key Word:
- 融合蛋白;增强型绿色荧光蛋白;Ano1;生理特性;钙激活Cl-通道;fusion protein;enhanced green fluorescent protein;Ano1;physiological feature;calcium-activated chloride channel
Abstract： 本研究旨在探讨融合在Ano1钙激活Cl-通道(anoctamin1)C末端的增强型绿色荧光蛋白(EGFP)对Ano1生理特性的影响.分别构建融合和未融合EGFP的Ano1真核表达载体,通过脂质体介导分别将这两类真核表达载体转染至Fischer大鼠甲状腺滤泡上皮(FRT)细胞,倒置荧光显微镜下观察融合和未融合EGFP的Ano1在FRT细胞中的表达与定位;荧光淬灭动力学检测Ano1转运I-的能力.结果显示,融合和未融合EGFP的Ano1均表达于FRT细胞膜上,且均能被Ca2+激活,融合和未融合EGFP的Ano1转运I-的能力无明显差异.以上结果提示,Ano1和EGFP-Ano1具有相似的生理特性.
- [1]Schroeder, BC,Jan, LY,Cheng, T,etc.Expression cloning of TMEM16A as a calcium-activated chloride channel subunit[J].2008,134(6).
- [2]Lee F. Greer III,Aladar A. Szalay.Imaging of light emission from the expression of luciferases in living cells and organisms: a review[J].2002,17(1).
- [3]Robert H. Newman,Jin Zhang,Vedangi Sample.The structure and function of fluorescent proteins[J].2009,38(10).
- [4]Arpino, James A. J.,Jones, D. Dafydd,Rizkallah, Pierre J..Structural and dynamic changes associated with beneficial engineered single-amino-acid deletion mutations in enhanced green fluorescent protein[J].2014,70(8).
- [5]Lambert, G.G.,Day, R.N.,Allen, J.R.,etc.A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum[J].2013,10(5).
- [6]Luis J.V Galietta,Peter M Haggie,A.S Verkman.Green fluorescent protein-based halide indicators with improved chloride and iodide affinities[J].2001,499(3).
- [7]Pan, Y.,Leifert, A.,Graf, M.,etc.High-sensitivity real-time analysis of nanoparticle toxicity in green fluorescent protein-expressing zebrafish[J].2013,9(6).
- [8].The photochemistry of fluorescent proteins: implications for their biological applications.[J].2009,38(10).
- [9]Galietta,L.J.V.,Verkman,A.S..Chloride channels as drug targets[J].2009,8(2).