You Position: Home > Paper
Involvement of protein kinase C in NMDAR-dependent long-term potentiation in rat amygdala
( views:457, downloads:12 )
- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 6
- DOI:
- 10.3321/j.issn:0371-0874.2008.06.008
- Key Word:
- 杏仁核;场电位;长时程增强;Ⅳ-甲基-D-天冬氨酸受体;蛋白激酶C
Abstract： 本文在人鼠杏仁核脑片卜刺激外囊(external capsule,EC),在基底外侧杏仁核(basolateral amygdala,BLA)记录场电位,观察能否在杏仁核诱导长时程增强(long-term potentiation,LTP),并探讨杏仁核LTP形成的可能机制.应用两串间隔10 min的θ频率波刺激EC,可见BLA的场电位明显增强,持续时间在30 min以L.EC-BLA的LTP表现为通路特异性,可被N-甲基-D-天冬氨酸受体(N-methyl-D-aspartate receptor,NMDAR)阻断剂D,L-2-氨基-5磷酸基戊睃(APV)阻断.在灌流液中加入蛋白激酶C(protein kinase C,PKC)抑制剂chelerythrine chloride,对BLA的基础场电位和配对脉冲比率(paired-pulse ratio,PPR)没有影响;但在chelerythrine chloride存在的情况下,两串θ频率波刺激不能诱导出LTP:若于两串高频刺激10 min后,在灌流液中加入chelerythrine chloride不能阻断BLA的LTP.上述结果表明,EC-BLA的LTP为NMDAR依赖性,PKC参与BLA的LTP诱导和早期维持.
- [1]胡志安,谭银玲,罗峻,等.长时程增强诱导和维持过程中海马CA1区神经细胞粘附分子蛋白水平与mRNA表达的变化[J].2004,(1).
- [2]Rosen JB,Wallace KJ.Neurotoxic lesions of the lateral nucleus of the amygdala decrease conditioned fear but not unconditioned fear of a predator odor: comparison with electrolytic lesions.[J].2001,21(10).
- [3]T V, Bliss,G L, Collingridge.A synaptic model of memory: long-term potentiation in the hippocampus.[J].1993,361(6407).
- [4]Swanson LW,Petrovich GD.What is the amygdala? (see comments)[J].1998,21(8).
- [5]Scott L, Rauch,Lisa M, Shin,Christopher I, Wright.Neuroimaging studies of amygdala function in anxiety disorders.[J].2003,985.
- [6]Bear MF,Malenka RC.LTP and LTD: an embarrassment of riches.[J].2004,44(1).
- [7]Y Y, Huang,E R, Kandel.Postsynaptic induction and PKA-dependent expression of LTP in the lateral amygdala.[J].1998,21(1).
- [8]Murray EA.The amygdala, reward and emotion.[J].2007,11(11).
- [9]Nicoll RA,Bredt DS.AMPA receptor trafficking at excitatory synapses.[J].2003,40(2).
- [10]Selcher JC,Mirnikjoo B,Paylor R,etc.A role for the beta isoform of protein kinase C in fear conditioning.[J].2000,20(16).
- [11]Collingridge GL,Isaac JT,Wang YT.Receptor trafficking and synaptic plasticity.[J].2004,5(12).
- [12]J E, LeDoux.Emotion circuits in the brain.[J].2000,23.
- [13]Citri A,Malenka RC.Synaptic plasticity: multiple forms, functions, and mechanisms.[J].2008,33(1).
- [14]S, Maren,M, Baudry.Properties and mechanisms of long-term synaptic plasticity in the mammalian brain: relationships to learning and memory.[J].1995,63(1).
- [15]McDonald AJ.Cortical pathways to the mammalian amygdala.[J].1998,55(3).
- [16]W C, Abraham,M F, Bear.Metaplasticity: the plasticity of synaptic plasticity.[J].1996,19(4).
- [17]Tim Otto,Howard Eichenbaum,Cynthia G. Wible,etc.Learning-related patterns of CA1 spike trains parallel stimulation parameters optimal for inducing hippocampal long-term potentiation[J].1991,1(2).
- [18]Hussain RJ,Carpenter DO.A Comparison of the Roles of Protein Kinase C in Long-Term Potentiation in Rat Hippocampal Areas CA1 and CA3.[J].2005,25(3).
- [19]Decorte L,Jaffard R,Calandreau L,etc.A different recruitment of the lateral and basolateral amygdala promotes contextual or elemental conditioned association in Pavlovian fear conditioning[J].2005,12(4).
- [20]Maren S,Frey KA,Corcoran KA.Protein synthesis in the amygdala, but not the auditory thalamus, is required for consolidation of Pavlovian fear conditioning in rats.[J].2003,18(11).
- [21]H T, Blair,G E, Schafe,E P, Bauer,etc.Synaptic plasticity in the lateral amygdala: a cellular hypothesis of fear conditioning.[J].1900,8.
- [22]Fox, R. J.,Sorenson, C. A..Bilateral lesions of the amygdala attenuate analgesia induced by diverse environmental challenges[J].1994,648(2).