You Position: Home > Paper
Proto-oncogene c-src regulates the viability of rat spermatogonial stem cells in vitro through phosphorylated signal transducer and activator of transcription-3
( views:91, downloads:12 )
- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 3
- DOI:
- 10.3321/j.issn:0371-0874.2008.03.012
- Key Word:
- 大鼠;精原干细胞;原癌基因c-src;信号转导与转录激活子-3
Abstract： 本文旨在研究原癌基因c-src在体外培养的9日龄大鼠精原干细胞中的表达及其与精原干细胞活性相关的信号通路.用MTT比色法观察反义c-src脱氧寡核苷酸(oligodeoxynucleotides, ODNs)对精原干细胞作用的量效及时效关系;用RT-PCR检测c-src mRNA的表达;用Western blot检测c-src表达产物pp60c-src和磷酸化的信号转导与转录激活子-3(phosphorylated signal transducer and activator of transcription. 3, p-STAT3)的表达.结果显示,与空白对照组相比,10μmol/L反义c-src ODNs作用12h后精原干细胞活性下降8.1%(P<0.05),且c-src mRNA表达明显下调;Western blot结果显示反义c-src ODNs组pp60c-src、p-STAT3蛋白表达与空白对照组相比分别下降了33.8%、45.3%(均P<0.01).以上结果提示,原癌基因c-src可能通过p-STAT3蛋白影响精原干细胞的活性.
- [1]李洪春,张光毅.脑缺血大鼠海马信号转导与转录激活子-3的激活及其调控[J].2003,(3).
- [2]张大雷,米玉玲,王凯明,等.卵泡刺激素和表皮生长因子对小鼠精原细胞增殖的影响[J].2007,(4).
- [3]R M, Kypta,Y, Goldberg,E T, Ulug,etc.Association between the PDGF receptor and members of the src family of tyrosine kinases.[J].1990,62(3).
- [4]N E, Kremer,G, D'Arcangelo,S M, Thomas,etc.Signal transduction by nerve growth factor and fibroblast growth factor in PC12 cells requires a sequence of src and ras actions.[J].1991,115.
- [5]W, Xu,S C, Harrison,M J, Eck.Three-dimensional structure of the tyrosine kinase c-Src.[J].1997,385(6617).
- [6]Kilkenny DM,Reich MB,Price J,etc.c-Src regulation of fibroblast growth factor-induced proliferation in murine embryonic fibroblasts.[J].2003,278(19).
- [7]D K, Luttrell,L M, Luttrell,S J, Parsons.Augmented mitogenic responsiveness to epidermal growth factor in murine fibroblasts that overexpress pp60c-src.[J].1988,8.
- [8]S, Tanaka,M, Amling,L, Neff,etc.c-Cbl is downstream of c-Src in a signalling pathway necessary for bone resorption.[J].1996,383(6600).
- [9]Yamamoto Y,Sakurai R,Sabe H,etc.Expression and subcellular distribution of the active form of c-Src tyrosine kinase in differentiating human endometrial stromal cells.[J].2002,8(12).
- [10]R L, Brinster,J W, Zimmermann.Spermatogenesis following male germ-cell transplantation.[J].1994,91.
- [11]P, Chaturvedi,M V, Reddy,E P, Reddy.Src kinases and not JAKs activate STATs during IL-3 induced myeloid cell proliferation.[J].1998,16(13).
- [12]Joly E,Buteau J,Foisy S,etc.Glucagon-like peptide 1 induces pancreatic beta-cell proliferation via transactivation of the epidermal growth factor receptor.[J].2003,52(1).
- [13]J A, Cooper,K L, Gould,C A, Cartwright,etc.Tyr527 is phosphorylated in pp60c-src: implications for regulation.[J].1986,231.
- [14]R L, Brinster,M R, Avarbock.Germline transmission of donor haplotype following spermatogonial transplantation.[J].1994,91.