Paper - WANFANG MED ONLINE

You Position: Home > Paper

The selective 5-HT1A receptor antagonist WAY-100635 increases neuronal activity of the basolateral nucleus of the amygdala in 6-hydroxydopamine-lesioned rats

( views:238, downloads:6 )

Author:: No author available

Journal Title:: ACTA PHYSIOLOGICA SINICA

Issue:: 2

DOI:: 10.3321/j.issn:0371-0874.2008.02.016

Key Word:: 基底外侧杏仁核复合体;5-HT1A受体;WAY-100635;6-羟多巴胺;帕金森病;电生理学;basolateral amygdala complex;5-HT1A receptor;WAY-100635;6-hydroxydopamine;Parkinson's disease;electrophysiology

Abstract： In the present study, extracellular recording was used to examine the neuronal activity of the basolateral nucleus (BL) of theamygdala and the effects of systemic administration of the selective 5-HT1A receptor antagonist WAY-100635 on the neuronal activityin the normal rats and rats with 6-hydroxydopamine (6-OHDA)-produced lesions in the substantia nigra pars compacta (SNc), Theresults showed that the firing rates of BL projection neurons and intemeurons were (0.39±0.04) Hz and (0.83±0.16) Hz in the normalrats, and (0.32±0.04) Hz and (0.53±0.12) Hz in 6-OHDA-lesioned rats. There was no significant difference in the firing rates of BLprojection neurons and interneurons between the normal and 6-OHDA-lesioned rats. In the normal rats, all BL projection neurons firedin burst; 94% of BL interneurons fired in burst and 6% fired irregularly. In 6-OHDA-lesioned rats, 85% of BL projection neurons displayed a burst fLdng pattern and 15% fired irregularly; 86% of BL intemeurons had a burst firing pattern and 14% fired irregularly.The distribution of firing patterns of projection neurons and interneurons in the BL in 6-OHDA-lesioned rats did not differ from thatin the normal rats. Systemic administration of WAY-100635 at 0.1 mg/kg body weight did not change the mean firing rates of projectionneurons and interneurons in the BL in both normal and 6-OHDA-lesioned rats, However, a higher dose of WAY-100635 at 0.5 mg/kgbody weight significantly decreased the mean firing rate of BL projection neurons from (0.43±0.07) to (0.15±0.02) Hz in the normalrats (P<0.01), but significantly increased the activity of BL projection neurons in 6-OHDA-lesioned rats from (0.37±0.08) to (0.69±0.18) Hz (P<0.004). The mean firing rates of BL interneurons in the normal and 6-OHDA-lesioned rats did not change after adminis-tration of a higher dose of WAY-100635 at 0.5 mg/kg body weight. These results demonstrate that the activity of BL neurons aftersubstantia nigra dopaminergic lesion in the SNc is regulated by activation of intrinsic and extrinsic inputs, and that 5-HT1A receptorssignificantly contribute to the regulation of the activity of BL projection neurons in both normal and 6-OHDA-lesioned rats. Furthermore,WAY-100635 induced an increase in the mean firing rate of projection neurons in the BL in 6-OHDA-lesioned rats, suggesting that5-HT1A receptor is likely to play a role in generating affective symptoms in Parkinson's disease.

Reference

[1]张巧俊,高蕊,刘健,等.帕金森病大鼠中缝背核5-羟色胺能神经元电活动的变化[J].2007,(2).
[2]Lees A,Turjanski N,Brooks D,etc.Depression in Parkinson's disease: loss of dopamine and noradrenaline innervation in the limbic system.[J].2005,128(Pt.6).
[3]E H, van Vulpen,R W, Verwer.Organization of projections from the mediodorsal nucleus of the thalamus to the basolateral complex of the amygdala in the rat.[J].1989,500(1-2).
[4]M. Gustavo Murer,Luis A. Riquelme,Kuei Y. Tseng and,etc.Substantia nigra pars reticulata single unit activity in normal and 60HDA-lesioned rats: Effects of intrastriatal apomorphine and subthalamic lesions[J].1997,27(4).
[5]C, Stein,H, Davidowa,D, Albrecht.5-HT(1A) receptor-mediated inhibition and 5-HT(2) as well as 5-HT(3) receptor-mediated excitation in different subdivisions of the rat amygdala.[J].2000,38(3).
[6]Bernard Scatton,France Javoy-Agid,Liliane Rouquier,etc.Reduction of cortical dopamine, noradrenaline, serotonin and their metabolites in Parkinson's disease[J].1983,275(2).
[7]Q.P. Ma,G.F. Yin,M.K. Ai,etc.Serotonergic projections from the nucleus raphe dorsalis to the amygdala in the rat[J].1991,134(1).
[8]Grace AA,Rosenkranz JA.Modulation of basolateral amygdala neuronal firing and afferent drive by dopamine receptor activation in vivo.[J].1999,19(24).
[9]Junque C,Tolosa E,Summerfield C,etc.Amygdalar and hippocampal MRI volumetric reductions in Parkinson's disease with dementia.[J].2005,20(5).
[10]G. M. Halliday,Y. W. Li,P. C. Blumbergs,etc.Neuropathology of immunohistochemically identified brainstem neurons in Parkinson's disease[J].1990,27(4).
[11]Koyama S,Matsumoto N,Murakami N,etc.Role of presynaptic 5-HT1A and 5-HT3 receptors in modulation of synaptic GABA transmission in dissociated rat basolateral amygdala neurons.[J].2002,72(4/5).
[12]J L, Cummings,D L, Masterman.Depression in patients with Parkinson's disease.[J].1999,14(9).
[13]S. Pirot,R. Godbout,J. Mantz,etc.Inhibitory effects of ventral tegmental area stimulation on the activity of prefrontal cortical neurons: Evidence for the involvement of both dopaminergic and GABAergic components[J].1992,49(4).
[14]R J, Scibilia,J E, Lachowicz,C D, Kilts.Topographic nonoverlapping distribution of D1 and D2 dopamine receptors in the amygdaloid nuclear complex of the rat brain.[J].1992,11(2).
[15]Morales M,Battenberg E,de Lecea L,etc.Cellular and subcellular immunolocalization of the type 3 serotonin receptor in the rat central nervous system.[J].1996,36(2).
[16]C M, Sinton,S L, Fallon.Electrophysiological evidence for a functional differentiation between subtypes of the 5-HT1 receptor.[J].1988,157(2-3).
[17]Grace AA,Rosenkranz JA ns.pitt.edu.Dopamine attenuates prefrontal cortical suppression of sensory inputs to the basolateral amygdala of rats.[J].2001,21(11).
[18]C.P. L-H. CHEN,J. T. ALDER,L. BRAY,etc.Post-Synaptic 5-HT1A and 5-HT2A Receptors Are Increased in Parkinson's Disease Neocortex[J].1998,861(1).
[19]Henderson JM,Halliday GM,Stimson E,etc.Clinical correlates of selective pathology in the amygdala of patients with Parkinson's disease.[J].2002,125(Pt.11).
[20]Muma NA,Mitrovic I,Gorman L,etc.6-Hydroxydopamine-Induced Lesions of Dopaminergic Neurons Alter the Function of Postsynaptic Cholinergic Neurons Without Changing Cytoskeletal Proteins.[J].2001,168(1).
[21]Camicioli R,Kaye JA,Glassberg K,etc.Parkinson's disease is associated with hippocampal atrophy.[J].2003,18(7).
[22]Verhey FR,Scholtissen B,Steinbusch HW,etc.Serotonergic mechanisms in Parkinson's disease: opposing results from preclinical and clinical data.[J].2006,113(1).
[23]Perra S,Pistis M,Gessa GL,etc.Cannabinoids modulate neuronal firing in the rat basolateral amygdala: evidence for CB1- and non-CB1-mediated actions.[J].2004,46(1).
[24]Fedrowitz M,Gernert M,Loscher W,etc.Altered spontaneous discharge rate and pattern of basal ganglia output neurons in the circling (ci2) rat mutant.[J].2003,118(3).
[25]Grace AA,Rosenkranz JA.Cellular mechanisms of infralimbic and prelimbic prefrontal cortical inhibition and dopaminergic modulation of basolateral amygdala neurons in vivo.[J].2002,22(1).
[26]Swanson LW,Petrovich GD.What is the amygdala? (see comments)[J].1998,21(8).
[27]Morilak, D. A.,Ciaranello, R. D..5-HT~2 receptor immunoreactivity on cholinergic neurons of the pontomesencephalic tegmentum shown by double immunofluorescence[J].1993,627(1).
[28]Fatiha Radja,Anne-Marie Laporte,Geneviève Daval,etc.Autoradiography of serotonin receptor subtypes in the central nervous system[J].1991,18(1).
[29]M, Brinley-Reed,A J, McDonald.Evidence that dopaminergic axons provide a dense innervation of specific neuronal subpopulations in the rat basolateral amygdala.[J].1999,850(1-2).
[30]Guo L,Mascagni F,Mcdonald AJ.Projections of the medial and lateral prefrontal cortices to the amygdala: a Phaseolus vulgaris leucoagglutinin study in the rat.[J].1996,71(1).
[31]D, Frechilla,A, Cobreros,L, Saldise,etc.Serotonin 5-HT(1A) receptor expression is selectively enhanced in the striosomal compartment of chronic parkinsonian monkeys.[J].2001,39(4).
[32]R. Spehlmann,K. Norcross.Decreased sensitivity of neurons in the basolateral amygdala to dopamine and noradrenaline lontophoresis after a kindling stimulus[J].1984,83(1).
[33]A J, McDonald,J C, Pearson.Coexistence of GABA and peptide immunoreactivity in non-pyramidal neurons of the basolateral amygdala.[J].1989,100(1-3).
[34]Brooks DJ,Doder M,Lees AJ,etc.Tremor in Parkinson's disease and serotonergic dysfunction: an 11C-WAY 100635 PET study.[J].2003,60(4).
[35]Rex Y. Wang,George K. Aghajanian.Inhibition of neurons in the amygdala by dorsal raphe stimulation: Mediation through a direct serotonergic pathway[J].1977,120(1).
[36]Wang PH,Wang Q,Wong PT,etc.Simvastatin reverses the downregulation of dopamine D(1) and D(2) receptor expression in the prefrontal cortex of 6-hydroxydopamine-induced Parkinsonian rats.[J].2005,1045(1).
[37]m.pnu.com,Hoffmann WE,Tetko IV,etc.Different tonic regulation of neuronal activity in the rat dorsal raphe and medial prefrontal cortex via 5-HT(1A) receptors.[J].2001,304(3).
[38]Everitt BJ,Cardinal RN,Parkinson JA,etc.Emotion and motivation: the role of the amygdala, ventral striatum, and prefrontal cortex.[J].2002,26(3).
[39]Pare D,Lang EJ.Similar inhibitory processes dominate the responses of cat lateral amygdaloid projection neurons to their various afferents.[J].1997,77(1).
[40]Gao WJ,Goldman-Rakic PS,Wang Y.Dopamine modulation of perisomatic and peridendritic inhibition in prefrontal cortex.[J].2003,23(5).
[41]Rainnie DG.Serotonergic modulation of neurotransmission in the rat basolateral amygdala.[J].1999,82(1).