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Changes of NMDA receptor expression in rat hippocampal formation after establishment of spatial memory by different training modes

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Author:: No author available

Journal Title:: ACTA PHYSIOLOGICA SINICA

Issue:: 5

DOI:: 10.3321/j.issn:0371-0874.2006.05.007

Key Word:: 空间学习记忆;水迷宫;免疫荧光;海马结构;NMDA受体

Abstract：短期强化训练能否建立可靠的空间长时记忆?用不同训练方式建立空间记忆后,大鼠海马结构NMDA受体的表达发生怎样的变化?目前尚未见明确报道.本研究应用Morris水迷宫方法分别采用以下模式对大鼠进行训练:空间长时记忆训练模式(LT组)、空间短时记忆训练模式(ST组)以及短期强化训练模式(SRT组),对不同训练模式建立的空间记忆进行了比较,应用免疫荧光组织化学方法检测各组大鼠海马结构NMDA/NR1受体表达的变化.结果表明,Morris水迷宫训练过程中,LT和SRT组大鼠寻找站台的平均潜伏期和策略均无显著性差异;记忆检测发现,除LT组大鼠在站台所在象限的停留时间明显长于SRT组大鼠外,两组大鼠寻找站台的潜伏期和策略以及穿越站台的次数均无显著性差异.ST组大鼠海马结构NMDA/NR1的免疫反应强度与对照组相比,无显著差异.但是,LT和SRT组大鼠海马CA1区锥体细胞层及齿状回的颗粒细胞层NMDA/NR1免疫荧光反应都明显增强,两组之间比较无显著差异,但是两组分别与对照组和ST组相比均有显著性差异.上述结果提示,短期强化训练可建立与长期训练基本相同的空间长时记忆.大鼠海马结构CA1区和齿状回NMDA受体表达的增加,可能是空间长时记忆形成的机制之一.

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