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Kv3.4 channel is involved in rat pulmonary vasoconstriction induced by 15-hydroxyeicosatetraenoic acid

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Author:
No author available
Journal Title:
ACTA PHYSIOLOGICA SINICA
Issue:
1
DOI:
10.3321/j.issn:0371-0874.2006.01.013
Key Word:
15-羟二十碳四烯酸;电压门控性钾通道亚型;肺动脉血管平滑肌细胞;缺氧肺动脉血管收缩

Abstract´╝Ü 通过组织浴槽血管环方法观察Kv3.4通道特异阻断剂BDS-Ⅰ对15-羟二十碳四烯酸(15-hydroxyeicosatetraenoic acid,15-HETE)收缩肺动脉血管的影响;通过酶法分离、培养Wistar大鼠肺动脉血管平滑肌细胞(pulmonary artery smoothmuscle cells,PASMCs),RT-PCR和Western blot技术观察15-HETE对大鼠PASMCs上Kv3.4通道表达的影响,以探讨Kv3.4通道在15-HETE收缩肺动脉过程中的作用.结果如下:(1)15-HETE以浓度依赖方式使肺动脉环张力增加,对缺氧组大鼠肺动脉环张力作用更为明显,与正常对照组相比差异显著;(2)除去肺动脉内皮后,15-HETE引起血管收缩的强度较内皮完整时增强,呈剂量依赖性收缩反应;(3)阻断Kv3.4通道可抑制15-HETE收缩肺动脉;(4)15-HETE下调PASMCs膜上Kv3.4通道mRNA及蛋白质表达.上述观察结果提示Ky3.4通道参与由15-HETE引起的缺氧肺动脉血管收缩(hypoxic pulmonary vasoconstriction,HPV).

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