You Position: Home > Paper
Effect of pravastatin on cholesteryl esters in foam cells and the relation with caveolin-1
( views:224, downloads:28 )
- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 1
- DOI:
- 10.3321/j.issn:0371-0874.2006.01.008
- Key Word:
- 普伐他汀;小凹蛋白-1;胆固醇酯;泡沫细胞;动脉粥样硬化
Abstract： 本文旨在观察普伐他汀对鼠源巨噬细胞性泡沫细胞内胆固醇酯含量的影响,探讨此作用与小凹蛋白-1的关系.采用体外培养的鼠源性巨噬细胞株作为研究对象,加入氧化低密度脂蛋白(oxidizedlow densitylipoprotein,ox-LDL)使其形成泡沫细胞,运用高效液相色谱测定细胞内胆固醇酯的改变,同时运用Western blot检测细胞中小凹蛋白-1的表达,并观察普伐他汀对细胞内胆固醇酯和小凹蛋白-1影响的量效和时效关系.结果显示:普伐他汀可明显降低泡沫细胞内的胆固醇酯与总胆固醇的比值,且在一定范围内呈剂量依赖性和时间依赖性.在泡沫细胞中加入普伐他汀后能够促进小凹蛋白-1的表达,呈剂量依赖性和时间依赖性.上述结果提示普伐他汀通过降低细胞内胆固醇酯的含量,减轻细胞泡沫化程度.普伐他汀的这一作用可能与促进小凹蛋白-1表达上调有关.
- [1]Y, Lange,T L, Steck.The role of intracellular cholesterol transport in cholesterol homeostasis.[J].1996,6(6).
- [2]J J, Boyle.Macrophage activation in atherosclerosis: pathogenesis and pharmacology of plaque rupture.[J].2005,3(1).
- [3]Michael Aviram,Gertrude Dankner,Uri Cogan,etc.Lovastatin inhibits low-density lipoprotein oxidation and alters its fluidity and uptake by macrophages: In vitro and in vivo studies[J].1992,41(3).
- [4]P, Cullen,M, Fobker,K, Tegelkamp,etc.An improved method for quantification of cholesterol and cholesteryl esters in human monocyte-derived macrophages by high performance liquid chromatography with identification of unassigned cholesteryl ester species by means of secondary ion mass spectrom[J].1997,38(2).
- [5]I, Maor,M, Aviram.Oxidized low density lipoprotein leads to macrophage accumulation of unesterified cholesterol as a result of lysosomal trapping of the lipoprotein hydrolyzed cholesteryl ester.[J].1994,35(5).
- [6]王佐,李全忠,杨向东,等.高效液相色谱分析氧化型低密度脂蛋白处理的U937细胞胞内胆固醇及胆固醇酯[J].1998,(4).
- [7]Carl J, Vaughan,Antonio M, Gotto.Update on statins: 2003.[J].2004,110(7).
- [8]O'Neil CH,Pickering JG,Argmann CA,etc.Regulation of macrophage cholesterol efflux through hydroxymethylglutaryl-CoA reductase inhibition[J].2005,280(23).
- [9]A, Hara,N S, Radin.Lipid extraction of tissues with a low-toxicity solvent.[J].1978,90(1).
- [10]Fu Y.,Hoang A.,Parton RG.,etc.Expression of caveolin-1 enhances cholesterol efflux in hepatic cells[J].2004,279(14).
- [11]P, Liu,W P, Li,T, Machleidt,etc.Identification of caveolin-1 in lipoprotein particles secreted by exocrine cells.[J].1999,1(6).
- [12]R, Ross.The pathogenesis of atherosclerosis: a perspective for the 1990s.[J].1993,362(6423).
- [13]Hugh C. Rayner,Terry Horsburgh,Sally L. Brown,etc.Receptor-Mediated Endocytosis of Low-Density Lipoprotein by Cultured Human Glomerular Cells[J].1990,55(3).
- [14]Han J.,Hajjar D. P.,Febbraio M.,etc.Native and Modified Low Density Lipoproteins Increase the Functional Expression of the Macrophage Class B Scavenger Receptor, CD36[J].1997.
- [15]Uittenbogaard A,Smart EJ.Palmitoylation of caveolin-1 is required for cholesterol binding, chaperone complex formation, and rapid transport of cholesterol to caveolae.[J].2000,275(33).
- [16]N, Umetani,Y, Kanayama,M, Okamura,etc.Lovastatin inhibits gene expression of type-I scavenger receptor in THP-1 human macrophages.[J].1996,1303(3).