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Long-term neurotoxic effects of MDMA result in cortical and hippocampal structural changes
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 1
- DOI:
- 10.3321/j.issn:0371-0874.2006.01.006
- Key Word:
- MDMA;神经毒性;内源性焦虑物质;5-HT转运体;胶质纤维酸性蛋白;神经末梢
Abstract： 通过短时间多次给药建立3,4-亚甲基二氧基甲基苯丙胺(3,4-methylenedioxymethamphetamine,MDMA)的神经毒性模型,将雄性Wistar大鼠随机分为对照组和实验组,实验组给予MDMA 10 mg/kg,每小时一次,共4次,即总量为40mg/kg,对照组给予等体积生理盐水.于末次给药后32周采用原位杂交检测5-HT转运体(serotonintransporter,SERT)mRNA和内源性焦虑物质苯甲二氮(艹卓)绪合性抑制物(diazepam binding inhibitor,DBI)的mRNA表达,免疫组织化学染色检测胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)的表达,银染观察神经末梢变化.结果显示,短时间多次给予MDMA后,与生理盐水组比较,MDMA组大鼠海马SERT mRNA信号表达降低(P＜0.05),大脑皮层DBI mRNA的信号表达增高(P＜0.05),GFAP表达显著升高(P＜0.05);银染MDMA组大鼠皮层神经末梢明显减少.上述结果提示,MDMA神经毒性导致皮层和海马结构改变持续存在,进而导致脑功能的紊乱.
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