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Dynamic changes in focal adhesion kinase during cell migration induced bybFGF and the significance
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 4
- DOI:
- 10.3321/j.issn:0371-0874.2004.04.015
- Key Word:
- 迁移;粘着斑激酶(FAK);碱性成纤维细胞生长因子(bFGF);整合素α3
Abstract： 本文旨在观察不同浓度碱性成纤维细胞生长因子(basicfibroblast growthfactor,bFGF)引起体外培养的ECV-304细胞迁移时粘着斑激酶(focal adhesion kinase,FAK)的动态变化及FAK与细胞迁移的关系.建立体外培养的ECV-304细胞划痕损伤模型,观察经不同剂量(0、5、10、15 ng/ml)bFGF作用12～24 h内细胞迁移距离(电脑图像测定)和FAK蛋白含量(Western blot)、活性(免疫沉淀加Western blot)和mRNA(RT-PCR)的动态变化.用免疫细胞化学(ABC法)染色研究整合素α3表达.结果发现,低浓度(5 ng/ml)bFGF促进细胞迁移,FAK蛋白含量增加42.07±2.02%、活性增加71.37±1.85%,与对照组比,差异显著(P＜0.05),并与迁移距离呈正相关(P＜0.05).高浓度(15 ng/ml)bFGF抑制细胞迁移,FAK的变化相反.FAK mRNA的变化比蛋白变化早出现6 h.与对照组比,各实验组整合素α3表达无明显差异.由此可见,不同剂量bFGF对ECV-304细胞迁移的双相调节作用与FAK含量、活性与mRNA表达呈正相关,FAK在bFGF引起的细胞迁移的信号转导途径中起着重要作用.
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