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Changes in antioxidant capacity of the guinea pig exposed to noise and the protective effect of α-lipoic acid against acoustic trauma
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 6
- DOI:
- 10.3321/j.issn:0371-0874.2003.06.009
- Key Word:
- 噪声性听力损伤;耳蜗;总抗氧化能力;一氧化氮;α-硫辛酸
Abstract： 实验探讨了声刺激后豚鼠血清总抗氧化能力(total antioxidant capacity,TAC)和耳蜗组织一氧化氮(nitric oxide,NO)含量的变化及α-硫辛酸的抗氧化和对声损伤的保护作用.将豚鼠(350-400g)随机分为无噪声对照组(n=20)、噪声+生理盐水组(n=20)和噪声+α-硫辛酸组(n=20).噪声刺激(4-kHz倍频程,115 dB SPL 5 h)结束后立即测试脑干诱发电位(auditory brainstem responses,ABRs),取血清测TAC,制备耳蜗组织匀浆测NO的水平.所得结果如下:(1)无噪声对照组,动物听阈无明显变化;噪声刺激后生理盐水组,听阈上升的幅度明显高于α-硫辛酸组(P＜0.05).(2)噪声+生理盐水组,TAC明显低于无噪声对照组(P＜0.05);噪声+α-硫辛酸组同噪声+生理盐水组相比,TAC明显升高(P＜0.05),同无噪声对照组相比,无显著性差异(P＞0.05).(3)噪声+生理盐水组,NO含量高于无噪声对照组(P＜0.05);噪声+α-硫辛酸组同噪声+生理盐水组相比,NO含量明显减少(P＜0.05),同无噪声对照组相比,差异无显著性(P＞0.05).上述结果提示,噪声刺激后血清TAC降低,耳蜗组织内NO含量增加;α-硫辛酸可通过抗氧化机制对噪声性听力损伤(noise induced hearing loss,NIHL)发挥保护作用.
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